EBK VISUAL ANATOMY & PHYSIOLOGY
EBK VISUAL ANATOMY & PHYSIOLOGY
3rd Edition
ISBN: 9780134454658
Author: Petti
Publisher: PEARSON CUSTOM PUB.(CONSIGNMENT)
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Chapter 5.2, Problem 17SR
Summary Introduction

To label: The structures of a hair follicle in the accompanying figure.

Introduction: Hair is a protein filament that grows from the follicles found in the dermis. Each hair is anchored by a hair follicle. Hair follicle is a dynamic structure that is located in within the dermis of the skin. It is made up of twenty different cell types with different functions.

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1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?  2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following:  Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng  Final recovery volume: 0.50 mL  Volume plated: 0.25 mL  Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?   2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?   3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…

Chapter 5 Solutions

EBK VISUAL ANATOMY & PHYSIOLOGY

Ch. 5.1 - Describe the main structural features of the...Ch. 5.1 - Prob. 3LOCh. 5.1 - Prob. 4LOCh. 5.1 - Prob. 5LOCh. 5.1 - Prob. 1ICh. 5.1 - Prob. 2ICh. 5.1 - Prob. 3ICh. 5.1 - Prob. 4ICh. 5.1 - Prob. 1SRCh. 5.1 - Short answer: Identify and describe the parts of...Ch. 5.1 - Prob. 16SRCh. 5.1 - Prob. 17SRCh. 5.2 - Prob. 1RCh. 5.2 - Prob. 2RCh. 5.2 - Prob. 3RCh. 5.2 - Prob. 4RCh. 5.2 - Prob. 5RCh. 5.2 - Prob. 6RCh. 5.2 - Prob. 7RCh. 5.2 - Prob. 8RCh. 5.2 - Prob. 9RCh. 5.2 - Prob. 10RCh. 5.2 - Prob. 11RCh. 5.2 - Prob. 12RCh. 5.2 - Prob. 1LOCh. 5.2 - Describe the mechanisms of hair production, and...Ch. 5.2 - Prob. 3LOCh. 5.2 - Prob. 4LOCh. 5.2 - Prob. 5LOCh. 5.2 - Describe the interaction between sunlight and...Ch. 5.2 - Prob. 7LOCh. 5.2 - Prob. 1ICh. 5.2 - Prob. 2ICh. 5.2 - Prob. 3ICh. 5.2 - Prob. 4ICh. 5.2 - Prob. 1SRCh. 5.2 - Prob. 2SRCh. 5.2 - Prob. 3SRCh. 5.2 - Prob. 4SRCh. 5.2 - Prob. 5SRCh. 5.2 - Prob. 6SRCh. 5.2 - Prob. 7SRCh. 5.2 - Prob. 8SRCh. 5.2 - Prob. 9SRCh. 5.2 - Prob. 10SRCh. 5.2 - Prob. 11SRCh. 5.2 - Prob. 12SRCh. 5.2 - Prob. 13SRCh. 5.2 - Prob. 14SRCh. 5.2 - Prob. 15SRCh. 5.2 - Prob. 16SRCh. 5.2 - Prob. 17SRCh. 5.2 - Prob. 18SRCh. 5.2 - Prob. 19SRCh. 5.2 - Prob. 20SRCh. 5.2 - Prob. 21SRCh. 5.2 - Prob. 22SRCh. 5.2 - Prob. 23SRCh. 5.2 - Prob. 24SRCh. 5.2 - Prob. 25SRCh. 5.2 - Prob. 26SRCh. 5.2 - Prob. 27SRCh. 5.2 - Prob. 28SRCh. 5.2 - Prob. 29SRCh. 5.2 - Prob. 30SRCh. 5.2 - Prob. 31SRCh. 5 - Prob. 1CRQCh. 5 - Prob. 2CRQCh. 5 - Prob. 3CRQCh. 5 - Prob. 4CRQCh. 5 - Prob. 5CRQCh. 5 - Prob. 6CRQCh. 5 - Prob. 7CRQCh. 5 - Prob. 8CRQCh. 5 - Prob. 9CRQCh. 5 - Prob. 10CRQCh. 5 - Prob. 11CRQCh. 5 - Prob. 12CRQCh. 5 - Prob. 13CRQCh. 5 - Which portion of the hair follicle produces the...Ch. 5 - Prob. 15CRQCh. 5 - Prob. 16CRQCh. 5 - Prob. 17CRQCh. 5 - Prob. 18CRQCh. 5 - Prob. 19CRQCh. 5 - Prob. 20CRQCh. 5 - Prob. 21CRQCh. 5 - Prob. 22CRQCh. 5 - Prob. 23CRQCh. 5 - Prob. 24CRQCh. 5 - Prob. 25CRQCh. 5 - Prob. 26CRQCh. 5 - Prob. 27CRQCh. 5 - Prob. 28CRQCh. 5 - Prob. 29CRQCh. 5 - Prob. 30CRQCh. 5 - Prob. 31CRQCh. 5 - Prob. 32CRQCh. 5 - Prob. 33CRQCh. 5 - Prob. 1CICh. 5 - Prob. 2CICh. 5 - Prob. 3CICh. 5 - Prob. 4CI
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