BIOCHEMISTRY (HARDBACK) W/ACCESS CODE
6th Edition
ISBN: 9781337194204
Author: GARRETT
Publisher: CENGAGE L
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Chapter 5, Problem 8P
Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.
- Solving the Sequence of an Oligopeptide From Sequence Analysis Data
Amino acid analysis of an oligopeptide containing nine residues revealed the presence of the following amino adds:
Arg Cys Gly Leu Met Pro Tyr Val
The following was found:
- Carboxypeptidase A treatment yielded no free amino add.
- Edman analysis of the intact oligopeptide released
c. Neither trypsin nor ehymotrypsin treatment of the nonapeptide released smaller fragments. However, combined trypsin and chymotrypsin treatment liberated free Arg.
- CNBr treatment of the eight-residue fragment left after combined trypsin and chymotrypsin action yielded a six-residue fragment containing Cys* Gly. Pro, Tyr, and Val and a dipeptide.
- Treatment of the six-residue fragment with β-mercaptoethanol yielded two tripeptidcs. Brief Edman analysis of the tripeplide mixture yielded only ΡÎ�Ή-Cys. (The sequence of each tripeptide, as read from the N-terminal end, is alphabetical if the one-lelter designation for amino acids is used.)
What is the amino acid sequence of this nonapeptide?
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12. When glutamic acid is heated to 180°C., it loses a molecule of water to form a
lactam. The bond that creates the lactam is an amide bond that forms between the
amino nitrogen and the carbon of the side chain -COOH group. What is the most
reasonable structure for this lactam? (NOTE: The structure of glutamic acid is shown
in Question 1.)
COOH
COOH
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C
D
COOH
B
COOH
E
COOH
10. Which one of the
following compounds is
the major organic product
of the series of reactions
shown here?
Ph
A
OH
Ph
B
CO₂Et
Br
-H
Ν ΚΑ
CO₂Et
1. NaOEt
1. NaOH, H₂O
2. H3O+
2. PhCH2CH2Br
3. heat
NH2
Ph
OH
NH2
0
OH
Ph
OH
NH2
Ph
D
NH2
E
OH
1. What is the isoelectric point of glutamic acid?
(Glutamic acid has pKa1 2.10, pKa2 4.07, pKaз 9.47)
A) pH 2.1
D) pH 6.8
B) pH 3.1
C) pH 4.1
E) pH 9.5
HO
NH2
Glutamic acid
(shown without charges)
OH
Chapter 5 Solutions
BIOCHEMISTRY (HARDBACK) W/ACCESS CODE
Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...
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