BIOCHEMISTRY (HARDBACK) W/ACCESS CODE
6th Edition
ISBN: 9781337194204
Author: GARRETT
Publisher: CENGAGE L
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Chapter 5, Problem 8P
Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.
- Solving the Sequence of an Oligopeptide From Sequence Analysis Data
Amino acid analysis of an oligopeptide containing nine residues revealed the presence of the following amino adds:
Arg Cys Gly Leu Met Pro Tyr Val
The following was found:
- Carboxypeptidase A treatment yielded no free amino add.
- Edman analysis of the intact oligopeptide released
c. Neither trypsin nor ehymotrypsin treatment of the nonapeptide released smaller fragments. However, combined trypsin and chymotrypsin treatment liberated free Arg.
- CNBr treatment of the eight-residue fragment left after combined trypsin and chymotrypsin action yielded a six-residue fragment containing Cys* Gly. Pro, Tyr, and Val and a dipeptide.
- Treatment of the six-residue fragment with β-mercaptoethanol yielded two tripeptidcs. Brief Edman analysis of the tripeplide mixture yielded only ΡÎ�Ή-Cys. (The sequence of each tripeptide, as read from the N-terminal end, is alphabetical if the one-lelter designation for amino acids is used.)
What is the amino acid sequence of this nonapeptide?
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Specific rotation is a measure of a solution's capacity to rotate circularly polarized light. The unfolding of the a helix of a
polypeptide to a random conformation is accompanied by a large decrease in specific rotation.
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pH to 7, there is a large decrease in the specific rotation of the solution. Similarly, polylysine (1.-Lys residues) is an a helix at pH
10, but when researchers lower the pH to 7 the specific rotation also decreases, as shown in the graph.
a Helix
Specific rotation
Poly(Glu)
a Helix
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conformation
Poly(Lys)
Random conformation
T
+
°
2
4
6 В
10 12 14
PH
Complete the statements about the molecular mechanism for these changes in specific rotation.
Increasing the pH of a polyglutamate solution from 6 to 7 causes the carboxyl group of each glutamate residue
Comed Artwer
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Chapter 5 Solutions
BIOCHEMISTRY (HARDBACK) W/ACCESS CODE
Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...
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