MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
4th Edition
ISBN: 9780393622805
Author: SLONCZEWSKI
Publisher: Norton custom
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Chapter 5, Problem 6RQ
Summary Introduction
To review:
The process through which the cells protect themselves from osmotic stress.
Introduction:
Cell is the smallest unit of life and it results in the formation of simple to complex organisms. The cell is covered by a cell membrane that separates the intracellular region with the extracellular region. Osmotic stress is a condition that results in a rapid change of water movement across the cell membrane while osmosis is a process where movement of the water molecules occur across a semi-permeable membrane from high concentration to low concentration.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 5 Solutions
MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
Ch. 5.2 - Prob. 1TQCh. 5.2 - Prob. 2TQCh. 5.2 - Prob. 3TQCh. 5.3 - Prob. 1TQCh. 5.4 - Prob. 1TQCh. 5.5 - Prob. 1TQCh. 5.5 - Prob. 2TQCh. 5.5 - Prob. 3TQCh. 5.6 - Prob. 1TQCh. 5.7 - Prob. 1TQ
Ch. 5.7 - Prob. 2TQCh. 5.7 - Prob. 3TQCh. 5 - Prob. 1RQCh. 5 - Prob. 2RQCh. 5 - Prob. 3RQCh. 5 - Prob. 4RQCh. 5 - Prob. 5RQCh. 5 - Prob. 6RQCh. 5 - Prob. 7RQCh. 5 - Prob. 8RQCh. 5 - Prob. 9RQCh. 5 - Prob. 10RQCh. 5 - Prob. 11RQCh. 5 - Prob. 12RQCh. 5 - Prob. 1TQCh. 5 - Prob. 2TQCh. 5 - Prob. 3TQCh. 5 - Prob. 4TQCh. 5 - Prob. 5TQ
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- During a routine medical check up of a healthy man it was found that his haematocrit value was highly unusual – value of 60%. What one of the options below is the most likely reason? He will have a diet high in iron. He is likely to be suffering from anaemia. He lives at high altitude. He has recently recovered from an accident where he lost a lot of blood. He has a very large body size.arrow_forwardExplain what age of culture is most likely to produce an endospore?arrow_forwardExplain why hot temperatures greater than 45 degrees celsius would not initiate the sporulation process in endospores?arrow_forward
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