Biochemistry
8th Edition
ISBN: 9781285429106
Author: Campbell, Mary K., FARRELL, Shawn O.
Publisher: Cengage Learning,
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Chapter 5, Problem 62RE
Interpretation Introduction
Interpretation:
The reason behind the statement, “the point to label a secondary antibody with a marker that can be visualized, instead of just labelling the primary antibody,” is to be defined.
Concept Information:
ELISA stands for the enzyme-linked immunosorbent assay. It is a technique used for the identification of proteins with the help of the interaction between the proteins and the antibodies.
In ELISA, the proteins to be identified are poured on a microtiter plate for their localization. Thereafter, an antibody (primary antibody, prepared against a specific protein) is introduced into the microtiter plate.
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Chapter 5 Solutions
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