Pathophysiology
Pathophysiology
6th Edition
ISBN: 9780323354813
Author: Jacquelyn L. Banasik PhD ARNP
Publisher: Saunders
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Chapter 5, Problem 5KQ
Summary Introduction

To determine: The mechanisms in which the cells containing identical genes in an organism become differentiated into divergent cell types.

Introduction: A process in which a cell gets changed from one type to another is referred to as cell differentiation. In this process, the cells become the specialized type of cells in order to perform a specific function. This is carried out through gene expression in which the combination of genes are expressed or repressed. On the basis of this expression, the cells obtain their specific function during cell differentiation.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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