Chapter 5, Problem 30CQ

(a)

Summary Introduction

To calculate: The molecular mass of the hen egg-white lysozyme (HEWL) for peaks 5 and 6 of ESI-MS spectrum.

Concept introduction: The proteins involves creating positively charged ions of the protein and separating them according to their mass to charge ratio (m/z) is called Electrospray ionization mass spectrometry (ESI-MS). It is the ionization technique to produce ions using an electrospray from macromolecules.

(b)

Summary Introduction

To calculate: The charge of the ion at peak 5 of the ESI-MS spectrum of the hen egg-white lysozyme (HEWL).

Concept introduction: The proteins involves creating positively charged ions of the protein and separating them according to their mass to charge ratio (m/z) is called Electrospray ionization mass spectrometry (ESI-MS). It is the ionization technique to produce ions using an electrospray from macromolecules.