BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
9th Edition
ISBN: 9781319425746
Author: BERG
Publisher: MAC HIGHER
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Chapter 5, Problem 2P
Interpretation Introduction
Interpretation:
The ovalbumin cDNA or ovalbumin genomic DNA should whether be used to form the protein in E.coli or not is to be stated. The reason corresponding to the correct choice is to be stated.
Concept introduction:
A segment which is located on hnRNA and cannot transfer genetic information is known as intron. A segement which is located on hnRNA and can encode single part of the mature RNA after eliminating the introns is known as exon.
The protein that is mainly found in egg white and shows the
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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.
The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
What is the formation of glycosylated hemoglobin (the basis for the HbA1c test)? Can you describe it?
Please analze the gel electrophoresis column of the VRK1 kinase (MW: 39.71 kDa).
Also use a ruler to measure the length of the column in centimeters and calculate the MW of each band observed.
Lane 1: buffer
Lane 2 : Ladder
Lane 3: Lysate
Lane 4: Flowthrough
Lane 5: Wash
Lanes 6-8: E1, E2, E3
Lane 9: Dialyzed VRK1
Lane 10: LDH
Chapter 5 Solutions
BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
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