FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
5th Edition
ISBN: 9781119498742
Author: Voet
Publisher: WILEY
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Chapter 5, Problem 26CQ
(a)
Summary Introduction
To derive: The degree of purification for a purified fraction.
Concept introduction: Sedimentation coefficient of a particle is used to characterize its behavior in the sedimentation processes, usually centrifugation. Ultracentrifugation is a technique that involves the application of centrifugal force to separate molecules from the solution, based on their viscosity of the medium, shape, density, size, and the rotor speed. Ammonium sulfate precipitation is the most common and effective method for fractionating proteins.
(b)
Summary Introduction
To derive: The percent of yield of enzyme recovered from the purified fraction.
Concept introduction: Sedimentation coefficient of a particle is used to characterize its behavior in the sedimentation processes, usually centrifugation. Ultracentrifugation is a technique that involves the application of centrifugal force to separate molecules from the solution, based on their viscosity of the medium, shape, density, size, and the rotor speed. Ammonium sulfate precipitation is the most common and effective method for fractionating proteins.
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Chapter 5 Solutions
FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
Ch. 5 - Prob. 1ECh. 5 - Prob. 2ECh. 5 - 3. Which peptide has greater absorbance at 280...Ch. 5 - 4. Protein X has an absorptivity of 0.4 mL · mg?1...Ch. 5 - 5. You are using ammonium sulfate to purify...Ch. 5 - Prob. 6ECh. 5 - 7. (a) In what order would the amino acids Arg,...Ch. 5 - Prob. 8ECh. 5 - 9. Explain why a certain protein has an apparent...Ch. 5 - 10. Determine the subunit composition of a protein...
Ch. 5 - 11. Explain why a protein, which has a...Ch. 5 - 12. A protein has an apparent mass of 800 kD by...Ch. 5 - 13. Explain why the dansyl chloride treatment of a...Ch. 5 - 14. Identify the tint residue obtained by Edman...Ch. 5 - 15. A pentapeptide has the sequence NNKNN (using...Ch. 5 - 16. The peptide in Problem 15 was sequenced by...Ch. 5 - 17. In site-directed mutagenesis experiments, Gly...Ch. 5 - 18. Below is a list of the first 10 residues of...Ch. 5 - Prob. 19ECh. 5 - Prob. 20ECh. 5 - Prob. 21CQCh. 5 - Prob. 22CQCh. 5 - 23. What fractionation procedure could be used to...Ch. 5 - Prob. 24CQCh. 5 - 25. Purification tables are often used to keep...Ch. 5 - Prob. 26CQCh. 5 - 27. You must cleave the following peptide into...Ch. 5 - 28. You wish to determine the sequence of a...Ch. 5 - Prob. 29CQCh. 5 - Prob. 30CQCh. 5 - 31. You wish to determine the sequence of a short...Ch. 5 - Prob. 32CQCh. 5 - Prob. 33CQCh. 5 - 34. You wish to sequence the light chain of a...Ch. 5 - Prob. 1MTE
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