Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 5, Problem 21P
Interpretation Introduction
Interpretation:
The reason corresponding to the given survey that the person B possesses no symptoms of drugs is to be stated. The possible defects in the other given people are to be stated.
Concept introduction:
The basic unit of heredity which is made up of DNA segments is known as gene.
The process of duplicating a specific DNA segment into a RNA segment with the help of an enzyme is known as transcription. The process of synthesizing proteins inside the cytoplasm after the completion of transcription is known as translation.
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Example 1:
1. Suppose an enzyme (MW = 5,000 g/mole) has a concentration of 0.05 mg/L. If the
kcat is 1 x 10 s, what is the theoretical maximum reaction velocity for the
enzyme?
A) 1050 µM/s.
B) 100 µM/s.
C) 150 μM/s.
D) 105 μM/s.
In 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat
liver. Their hypothesis was that phosphocholine joined with some cellular component to yield lecithin. In an earlier experiment,
incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled
lecithin. This became their assay for the enzymes involved in lecithin synthesis.
Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values.
-O-P-O-CH2-CH₁₂-N(CH3)3
Phosphocholine
H₂C-O-C-R
HC-O-C-R2
+
+
+
Cell fraction + ?
HC-O-P-O-CH₁₂-CH₂-N(CH),
O
Phosphatidylcholine
The researchers then centrifuged the broken cell preparation
to separate the membranes from the soluble proteins. They
tested three preparations: whole extract, membranes, and
soluble proteins. Table 1 summarizes the results.
Table 1: Cell fraction requirement for incorporation of
32p-phosphocholine into…
Researchers isolated an unknown substance, X, from rabbit muscle. They determined its structure from the following
observations and experiments.
(a) Qualitative analysis showed that X was composed entirely of C, H, and O. A weighed sample of X was completely oxidized
and the H2O and CO2 produced were measured. This quantitative analysis revealed that X contained 40.00% C, 6.71% H, and
53.29% O by weight.
(b) The molecular mass of X, as determined by mass spectrometry, was 90.00 atomic mass units (u).
(c) Infrared spectroscopy showed that X contained one double bond.
(d) X dissolved readily in water, and the solution demonstrated optical activity when tested in a polarimeter.
(e) The aqueous solution of X is acidic.
What is the empirical formula of X?
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