The chains in the three-dimensional model correspond to the antibody fragment, and which correspond to the antigen, lysozyme. Introduction : Antibodies are the Y-shaped proteins which provide immunity to the body by neutralizing antigens such as bacteria and viruses. They are also known as immunoglobulins. On the other hand, an antigen is a foreign particle that induces the production of antibodies. These protein structures are the three-dimensional arrangement of atoms in a peptide chain.

Question

Want to see the full answer?

Answer 1

Question

Chapter 5, Problem 16BO

(a)

Summary Introduction

To determine: The chains in the three-dimensional model correspond to the antibody fragment, and which correspond to the antigen, lysozyme.

Introduction:

Antibodies are the Y-shaped proteins which provide immunity to the body by neutralizing antigens such as bacteria and viruses. They are also known as immunoglobulins. On the other hand, an antigen is a foreign particle that induces the production of antibodies. These protein structures are the three-dimensional arrangement of atoms in a peptide chain.

(b)

Summary Introduction

To determine: The type of secondary structure that predominates in the Fab fragment.

Introduction:

An antibody is divided into two regions, Fab (antigen-binding) fragment and Fc (crystallizable) fragment. Fab fragment is a portion of an antibody molecule that binds to the antigen molecule.

(c)

Summary Introduction

To determine: The number of amino acid residues in the heavy and light chains of the Fab fragmentand also estimate the percentage of the lysozyme that interacts with the antigen binding site of the antibody fragments.

Introduction:

An antibody has a Y-shaped structure which is formed by the association of heavy and light chain. Both the chains contain an amino-terminal at the Fab portion, and a carboxy terminal at the Fc portion.

(d)

Summary Introduction

To determine: The specific amino acid residues in lysozyme and in variable regions of the Fab heavy and light chains that are situated at the antigen-antibody interface. Also determine whether the amino acid residues are contiguous in the primary sequence of the polypeptide chains.

Introduction:

Amino acid residue is the fundamental unit of a protein. Two or three amino acid residues are bonded together by peptide bond to form a peptide. A long chain of peptides called a polypeptide is linked through the peptide bonds resulting in a specific protein molecule.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.

You were given a mixture of two proteins with different isoelectric points and molecular weights:• Protein X: pI 4.2, MW 42,000• Protein Y: pI 9.8, MW 90,000Using a Tris-glycine discontinuous native gel (pH8.3) electrophoresis system with a running gel of 12%, only a single band was observed upon protein staining after electrophoresis. Explain the observed result and discuss possible factors affecting protein migration in this system.

The standard cost of Product B manufactured by Oriole Company includes 3.5 units of direct materials at $5.40 per unit. During June, 27,300 units of direct materials are purchased at a cost of $5.15 per unit, and 27,300 units of direct materials are used to produce 7,600 units of Product B. (a) Compute the total materials variance and the price and quantity variances. Total materials variance Materials price variance Materials quantity variance $ (b) Compute the total materials variance and the price and quantity variances, assuming the purchase price is $6.35 and the quantity purchased and used is 26,300 units. Total materials variance Materials price variance Materials quantity variance $

Answer 2

Question

Chapter 5, Problem 16BO

(a)

Summary Introduction

To determine: The chains in the three-dimensional model correspond to the antibody fragment, and which correspond to the antigen, lysozyme.

Introduction:

Antibodies are the Y-shaped proteins which provide immunity to the body by neutralizing antigens such as bacteria and viruses. They are also known as immunoglobulins. On the other hand, an antigen is a foreign particle that induces the production of antibodies. These protein structures are the three-dimensional arrangement of atoms in a peptide chain.

(b)

Summary Introduction

To determine: The type of secondary structure that predominates in the Fab fragment.

Introduction:

An antibody is divided into two regions, Fab (antigen-binding) fragment and Fc (crystallizable) fragment. Fab fragment is a portion of an antibody molecule that binds to the antigen molecule.

(c)

Summary Introduction

To determine: The number of amino acid residues in the heavy and light chains of the Fab fragmentand also estimate the percentage of the lysozyme that interacts with the antigen binding site of the antibody fragments.

Introduction:

An antibody has a Y-shaped structure which is formed by the association of heavy and light chain. Both the chains contain an amino-terminal at the Fab portion, and a carboxy terminal at the Fc portion.

(d)

Summary Introduction

To determine: The specific amino acid residues in lysozyme and in variable regions of the Fab heavy and light chains that are situated at the antigen-antibody interface. Also determine whether the amino acid residues are contiguous in the primary sequence of the polypeptide chains.

Introduction:

Amino acid residue is the fundamental unit of a protein. Two or three amino acid residues are bonded together by peptide bond to form a peptide. A long chain of peptides called a polypeptide is linked through the peptide bonds resulting in a specific protein molecule.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 5, Problem 16BO

(a)

Summary Introduction

To determine: The chains in the three-dimensional model correspond to the antibody fragment, and which correspond to the antigen, lysozyme.

Introduction:

Antibodies are the Y-shaped proteins which provide immunity to the body by neutralizing antigens such as bacteria and viruses. They are also known as immunoglobulins. On the other hand, an antigen is a foreign particle that induces the production of antibodies. These protein structures are the three-dimensional arrangement of atoms in a peptide chain.

(b)

Summary Introduction

To determine: The type of secondary structure that predominates in the Fab fragment.

Introduction:

An antibody is divided into two regions, Fab (antigen-binding) fragment and Fc (crystallizable) fragment. Fab fragment is a portion of an antibody molecule that binds to the antigen molecule.

(c)

Summary Introduction

To determine: The number of amino acid residues in the heavy and light chains of the Fab fragmentand also estimate the percentage of the lysozyme that interacts with the antigen binding site of the antibody fragments.

Introduction:

An antibody has a Y-shaped structure which is formed by the association of heavy and light chain. Both the chains contain an amino-terminal at the Fab portion, and a carboxy terminal at the Fc portion.

(d)

Summary Introduction

To determine: The specific amino acid residues in lysozyme and in variable regions of the Fab heavy and light chains that are situated at the antigen-antibody interface. Also determine whether the amino acid residues are contiguous in the primary sequence of the polypeptide chains.

Introduction:

Amino acid residue is the fundamental unit of a protein. Two or three amino acid residues are bonded together by peptide bond to form a peptide. A long chain of peptides called a polypeptide is linked through the peptide bonds resulting in a specific protein molecule.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 5, Problem 16BO

(a)

Summary Introduction

To determine: The chains in the three-dimensional model correspond to the antibody fragment, and which correspond to the antigen, lysozyme.

Introduction:

Antibodies are the Y-shaped proteins which provide immunity to the body by neutralizing antigens such as bacteria and viruses. They are also known as immunoglobulins. On the other hand, an antigen is a foreign particle that induces the production of antibodies. These protein structures are the three-dimensional arrangement of atoms in a peptide chain.

(b)

Summary Introduction

To determine: The type of secondary structure that predominates in the Fab fragment.

Introduction:

An antibody is divided into two regions, Fab (antigen-binding) fragment and Fc (crystallizable) fragment. Fab fragment is a portion of an antibody molecule that binds to the antigen molecule.

(c)

Summary Introduction

To determine: The number of amino acid residues in the heavy and light chains of the Fab fragmentand also estimate the percentage of the lysozyme that interacts with the antigen binding site of the antibody fragments.

Introduction:

An antibody has a Y-shaped structure which is formed by the association of heavy and light chain. Both the chains contain an amino-terminal at the Fab portion, and a carboxy terminal at the Fc portion.

(d)

Summary Introduction

To determine: The specific amino acid residues in lysozyme and in variable regions of the Fab heavy and light chains that are situated at the antigen-antibody interface. Also determine whether the amino acid residues are contiguous in the primary sequence of the polypeptide chains.

Introduction:

Amino acid residue is the fundamental unit of a protein. Two or three amino acid residues are bonded together by peptide bond to form a peptide. A long chain of peptides called a polypeptide is linked through the peptide bonds resulting in a specific protein molecule.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 5, Problem 16BO

(a)

Summary Introduction

To determine: The chains in the three-dimensional model correspond to the antibody fragment, and which correspond to the antigen, lysozyme.

Introduction:

Antibodies are the Y-shaped proteins which provide immunity to the body by neutralizing antigens such as bacteria and viruses. They are also known as immunoglobulins. On the other hand, an antigen is a foreign particle that induces the production of antibodies. These protein structures are the three-dimensional arrangement of atoms in a peptide chain.

(b)

Summary Introduction

To determine: The type of secondary structure that predominates in the Fab fragment.

Introduction:

An antibody is divided into two regions, Fab (antigen-binding) fragment and Fc (crystallizable) fragment. Fab fragment is a portion of an antibody molecule that binds to the antigen molecule.

(c)

Summary Introduction

To determine: The number of amino acid residues in the heavy and light chains of the Fab fragmentand also estimate the percentage of the lysozyme that interacts with the antigen binding site of the antibody fragments.

Introduction:

An antibody has a Y-shaped structure which is formed by the association of heavy and light chain. Both the chains contain an amino-terminal at the Fab portion, and a carboxy terminal at the Fc portion.

(d)

Summary Introduction

To determine: The specific amino acid residues in lysozyme and in variable regions of the Fab heavy and light chains that are situated at the antigen-antibody interface. Also determine whether the amino acid residues are contiguous in the primary sequence of the polypeptide chains.

Introduction:

Amino acid residue is the fundamental unit of a protein. Two or three amino acid residues are bonded together by peptide bond to form a peptide. A long chain of peptides called a polypeptide is linked through the peptide bonds resulting in a specific protein molecule.

Answer 6

Chapter 5, Problem 16BO

(a)

Summary Introduction

To determine: The chains in the three-dimensional model correspond to the antibody fragment, and which correspond to the antigen, lysozyme.

Introduction:

Antibodies are the Y-shaped proteins which provide immunity to the body by neutralizing antigens such as bacteria and viruses. They are also known as immunoglobulins. On the other hand, an antigen is a foreign particle that induces the production of antibodies. These protein structures are the three-dimensional arrangement of atoms in a peptide chain.

Answer 7

Chapter 5, Problem 16BO

(a)

Summary Introduction

To determine: The chains in the three-dimensional model correspond to the antibody fragment, and which correspond to the antigen, lysozyme.

Introduction:

Antibodies are the Y-shaped proteins which provide immunity to the body by neutralizing antigens such as bacteria and viruses. They are also known as immunoglobulins. On the other hand, an antigen is a foreign particle that induces the production of antibodies. These protein structures are the three-dimensional arrangement of atoms in a peptide chain.

Answer 8

(b)

Summary Introduction

To determine: The type of secondary structure that predominates in the Fab fragment.

Introduction:

An antibody is divided into two regions, Fab (antigen-binding) fragment and Fc (crystallizable) fragment. Fab fragment is a portion of an antibody molecule that binds to the antigen molecule.

Answer 9

(b)

Summary Introduction

To determine: The type of secondary structure that predominates in the Fab fragment.

Introduction:

An antibody is divided into two regions, Fab (antigen-binding) fragment and Fc (crystallizable) fragment. Fab fragment is a portion of an antibody molecule that binds to the antigen molecule.

Answer 10

(c)

Summary Introduction

To determine: The number of amino acid residues in the heavy and light chains of the Fab fragmentand also estimate the percentage of the lysozyme that interacts with the antigen binding site of the antibody fragments.

Introduction:

An antibody has a Y-shaped structure which is formed by the association of heavy and light chain. Both the chains contain an amino-terminal at the Fab portion, and a carboxy terminal at the Fc portion.

Answer 11

(c)

Summary Introduction

To determine: The number of amino acid residues in the heavy and light chains of the Fab fragmentand also estimate the percentage of the lysozyme that interacts with the antigen binding site of the antibody fragments.

Introduction:

An antibody has a Y-shaped structure which is formed by the association of heavy and light chain. Both the chains contain an amino-terminal at the Fab portion, and a carboxy terminal at the Fc portion.

Answer 12

(d)

Summary Introduction

To determine: The specific amino acid residues in lysozyme and in variable regions of the Fab heavy and light chains that are situated at the antigen-antibody interface. Also determine whether the amino acid residues are contiguous in the primary sequence of the polypeptide chains.

Introduction:

Amino acid residue is the fundamental unit of a protein. Two or three amino acid residues are bonded together by peptide bond to form a peptide. A long chain of peptides called a polypeptide is linked through the peptide bonds resulting in a specific protein molecule.

Answer 13

(d)

Summary Introduction

To determine: The specific amino acid residues in lysozyme and in variable regions of the Fab heavy and light chains that are situated at the antigen-antibody interface. Also determine whether the amino acid residues are contiguous in the primary sequence of the polypeptide chains.

Introduction:

Amino acid residue is the fundamental unit of a protein. Two or three amino acid residues are bonded together by peptide bond to form a peptide. A long chain of peptides called a polypeptide is linked through the peptide bonds resulting in a specific protein molecule.

Answer 14

Expert Solution & Answer

Answer 15

Expert Solution & Answer

Answer 16

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 17

Ch. 5 - Prob. 1P Ch. 5 - Prob. 2P Ch. 5 - Prob. 3P Ch. 5 - Prob. 4P Ch. 5 - Prob. 5P Ch. 5 - Prob. 6P Ch. 5 - Prob. 7P Ch. 5 - Prob. 8P Ch. 5 - Prob. 9P Ch. 5 - Prob. 10P

Concept explainers

Videos

To determine: The type of secondary structure that predominates in the Fab fragment.

Introduction:

An antibody is divided into two regions, Fab (antigen-binding) fragment and Fc (crystallizable) fragment. Fab fragment is a portion of an antibody molecule that binds to the antigen molecule.

Want to see the full answer?

Chapter 5 Solutions

Concept explainers

Videos

To determine: The type of secondary structure that predominates in the Fab fragment. Introduction: An antibody is divided into two regions, Fab (antigen-binding) fragment and Fc (crystallizable) fragment. Fab fragment is a portion of an antibody molecule that binds to the antigen molecule.

Want to see the full answer?

Chapter 5 Solutions

To determine: The type of secondary structure that predominates in the Fab fragment.

Introduction:

An antibody is divided into two regions, Fab (antigen-binding) fragment and Fc (crystallizable) fragment. Fab fragment is a portion of an antibody molecule that binds to the antigen molecule.