BIOCHEMISTRY
BIOCHEMISTRY
8th Edition
ISBN: 9781319296186
Author: BERG
Publisher: MAC HIGHER
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Chapter 5, Problem 15P
Interpretation Introduction

Interpretation:

Among the amino acid sequences, the sequence that would yield the most optimal oligonucleotide probe is to be predicted.

Concept introduction:

A small chain of single stranded RNA or DNA which is used to determine the presence of complementary sequence of nucleic acids with the help of hybridization is known as oligonuceotide probe. This complementary sequence of nucleic acids is also called the target sequence.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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