BIOCHEMISTRY 2 TERM ACCESS
9th Edition
ISBN: 9781319402877
Author: BERG
Publisher: MAC HIGHER
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Chapter 5, Problem 11P
Interpretation Introduction
Interpretation:
The reason corresponding to the fact that the given mutants of S. pyogenes Cas9 might be useful for genome editing studies is to be stated.
Concept introduction:
The full form of PAM sequence is Protospacer Adjacent Motif sequence. It is a small DNA sequence that contains approximately six base pairs lengthwise. The CRISPR-Cas9 targets the DNA region of PAM sequence for cleavage.
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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.
The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
Chapter 5 Solutions
BIOCHEMISTRY 2 TERM ACCESS
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