Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 49, Problem 2CT
Summary Introduction
To determine:
The role of antibiotics in urinary tract infections caused by yeast.
Introduction:
Antibiotics are antimicrobial medicines that help in stopping bacterial infections. They work by killing bacteria or inhibit them to reproduce, grow, and proliferate. The term antibiotic means "against life".
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Your goal is to produce black seeds resistant to mold. So you make the same cross again (between a homozygous black seeded, mold susceptible parent and a homozygous white seeded and mold resistant parent), and, again, advance progeny by SSD to create 100 F10 generation plants.
Based on the information you obtained from your first crossing experiment (Question #4), how many F10 plants would you expect to have black seeds and be resistant to mold?
Assume that a toxin produced by the mold fungus has been isolated. Only mold resistant seeds will germinate in the presence of the toxin. Could you use this toxin screening procedure to have segregation distortion work in your favor in the F2 generation? Explain your answer.
Info from Question 4
a. P Locus (Seed Color):
Hypothesis: The null hypothesis (H₀) is that seed color is controlled by alleles at a single locus.
Observed Data:
Total white seeds: 45 (resistant plants) + 6 (susceptible plants) = 51
Total black seeds: 7 (resistant…
10. Consider the following enzyme and its substrate where the "+" and "-" indicate cations and
anions, respectively. Explain which of the following inhibitors could inhibit this enzyme?
Which type of inhibitor would it be and why? (Video 5-2)
Substrate
Enzyme
Potential inhibitors
Using Punnett Squares Punnett squares are one good
way to predict the outcome of genetic crosses. Punnett
squares use mathematical probability to help predict the
genotype and phenotype combinations in genetic crosses. The
number of possible alleles from each parent determines the
number of rows and columns in the Punnett square.
Independent Assortment
KEY QUESTION How do alleles segregate when more than
one gene is involved?
Mendel wondered if the segregation of one pair of alleles
affects another pair. For example, does the gene that
determines the shape of a seed affect the gene for seed color?
This type of experiment is known as a two-factor, or dihybrid,
cross because it involves two different genes. Single-gene
crosses are monohybrid crosses.
Visual Reading Tool: Two-Factor Cross: F₂
The Punnett square shows the
results of self-crossing the F,
generation of a cross between
round yellow peas and wrinkled
green peas.
1. List the different genotypes in
the F, generation. What is the…
Chapter 49 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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- CHAPTER 12 LESSON 2 Applying Mendel's Principles READING TOOL Connect to Visuals Before you read, preview Figure 12-7. Try to infer the purpose of this diagram. As you read, compare your inference to the text. After you read, revise your statement if needed or write a new one about the diagram's purpose. Take notes on the lines provided. Then view the Punnett square and answer the questions below regarding the genotypes and phenotypes. Inference: Revision: Parent 2 rryy Gametes F ry Parent 1 RRYY Gametes RY RrYy The F, generation are all RrYy. 1. What is the phenotype of parent 1?. 2. What is the genotype of parent 1? 3. What is the phenotype of parent 2? 4. What is the genotype of parent 2? 5. What is the phenotype of the F, offspring?. 6. What is the genotype of the F, offspring?. 7. What kind of cross does this figure describe? 144 Chanter 12 Introduction to Genetice Copyright Pearson Education Inc. or its affiliator. All rights reserved.arrow_forwardHow is the term enzyme related to the term proteinarrow_forwardCan very low temperatures cause proteins to denature? Explain why or why not?arrow_forward
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- Linkage in common bean Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible. Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population…arrow_forwardAlleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible. Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population produced using single seed…arrow_forwardcan you help? I think its B but not surearrow_forward
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