Principles of Biology
Principles of Biology
2nd Edition
ISBN: 9781260081848
Author: Robert Brooker
Publisher: Mcgraw-hill Higher Education (us)
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Chapter 47, Problem 2TY
Summary Introduction

Introduction:

Biological diversity, biodiversity, is the variations in different species and the ecosystems in which these species survive. Biodiversity is studied in three stages, species diversity, genetic diversity, and ecosystem diversity.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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