Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 46, Problem 1CA
A 45-year-old man was admitted to the hospital with chest and back pain. On examination, his chest was dull to percussion. A chest X-ray film showed lower-left-lung infiltrates. A sputum culture revealed alpha-hemolytic, gram-positive, catalase-negative cocci that were inhibited by optochin. The bacteria produced acid from lactose. Use Appendix H to identify the species of this bacterium.
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What is this organism? And what other test could be done to confirm it's identification?
1.Gram stain - positive cocci, chains
Catalase - weak positive
Hemolysis - beta
BE - positive
NaCl- positive
Bacitracin - no zone of inhibition
PYR - positive
Answer: Enterococcus spp. CAMP test to verify the identity
2.Gram positive cocci, chains
Catalase - negative
Hemolysis - alpha
BE - negative
NaCl - negative
P disk - resistant
Bile Solubility - negative
Answer - viridans group, Use PYR test to verify
List the following to determine the unknown bacteria: Streptococcus mutans
1) Morphology & Characteristics
2) the different biological tests to be done
This is a catalase-positive, coagulase-negative, gram-positive coccus isolated from a urine specimen from a 20-year-old female college student. The image shown is a Mueller Hinton plate streaked with a 0.5 MacFarland standardized inoculum and a 5 microgram disk of novobiocin after overnight incubation. What is the identification of the isolate?
Please select the single best answer
Staphylococcus epidermidis
Staphylococcus aureus
Staphylococcus saprophyticus
Staphylococcus lugdenensis
Chapter 46 Solutions
Laboratory Experiments in Microbiology (11th Edition)
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