Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 44, Problem 2CA
Summary Introduction
To analyze:
The reason for using ELISA for detecting virus or viral antigens.
Introduction:
ELISA −Enzyme Linked Immunosorbent Assay is the immuno assay used for detecting suspected antigens for specific antibodies or for detecting suspected antibodies in a sample. Here the antigen is coated on to the well and the primary antibody is added. If there is specificity an antigen-antibody interaction takes place. Then another enzyme labelled secondary antibody is added to which the substrate specific to the enzyme is introduced. If there is enzyme substrate complex formation, then a coloured product is formed. The intensity of the colour change is directly proportional to the amount of antibodies present.
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