PRESCOTT'S MICROBIOLOGY
PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Chapter 43.2, Problem 2MI
Summary Introduction

Wastewater treatment by biological way is an important and integral treatment processes that treat soluble organic impurities or a mix of two different wastewater either from industry or municipalities. Aerobic activated sludge process is a well known biological treatment process that has been in practice over a century. The implementation of a variety of advanced biological treatment processes in recent years is due to increasing pressure to meet more stringent discharge standards or not being allowed to treated effluent discharge in the environment.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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