Campbell Biology Custom Stony Brook 10 Th Edition
10th Edition
ISBN: 9781269870818
Author: Reece Urry Cain Wasserman Minorsky Jackson
Publisher: PEARSON
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Question
Chapter 42.3, Problem 2CC
Summary Introduction
To explain: The short-term variations in cardiovascular functions that help an animal to use skeletal muscles to run away from danger.
Concept introduction:
A skeleton can support an animal, protect its internal organs, and allows for the movement of an organism. There are three types of skeletons. They are hydrostatic skeleton, exoskeleton, and endoskeleton.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
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Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 42 Solutions
Campbell Biology Custom Stony Brook 10 Th Edition
Ch. 42.1 - Prob. 1CCCh. 42.1 - Three-chambered hearts with incomplete septa were...Ch. 42.1 - Prob. 3CCCh. 42.2 - Explain why blood has a higher 02 concentration in...Ch. 42.2 - Prob. 2CCCh. 42.2 - Prob. 3CCCh. 42.3 - Prob. 1CCCh. 42.3 - Prob. 2CCCh. 42.3 - Prob. 3CCCh. 42.4 - Explain why a physician might order a white cell...
Ch. 42.4 - Prob. 2CCCh. 42.4 - Prob. 3CCCh. 42.4 - Prob. 4CCCh. 42.4 - Prob. 5CCCh. 42.5 - Why is an internal location for gas exchange...Ch. 42.5 - Prob. 2CCCh. 42.5 - MAKE CONNECTIONS Describe similarities in the...Ch. 42.6 - Prob. 1CCCh. 42.6 - Prob. 2CCCh. 42.6 - Prob. 3CCCh. 42.7 - Prob. 1CCCh. 42.7 - How does the Bohr shift help deliver O2 to very...Ch. 42.7 - Prob. 3CCCh. 42 - How does the flow of a fluid in a closed...Ch. 42 - Prob. 42.2CRCh. 42 - Prob. 42.3CRCh. 42 - Prob. 42.4CRCh. 42 - Prob. 42.5CRCh. 42 - How does air in the lungs differ from the fresh...Ch. 42 - How are the roles of a respiratory pigment and an...Ch. 42 - Prob. 1TYUCh. 42 - Blood returning to the mammalian heart in a...Ch. 42 - Prob. 3TYUCh. 42 - When you hold your breath, which of the following...Ch. 42 - Prob. 5TYUCh. 42 - Prob. 6TYUCh. 42 - Prob. 7TYUCh. 42 - DRAW IT Plot blood pressure against time for one...Ch. 42 - EVOLUTION CONNECTION One opponent of the movie...Ch. 42 - Prob. 10TYUCh. 42 - Prob. 11TYUCh. 42 - WRITE ABOUT A THEME: INTERACTIONS Some athletes...Ch. 42 - Prob. 13TYU
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