The terms negative feedback loop and positive feedback loop, and how they differ. Introduction: In living organisms, maintenance of a steady condition is important to carry out the required metabolic and physiological processes. This steady state, called homeostasis, is maintained through a feedback system in the body. This feedback system is also used to maintain the conditions in the required range during any biological process.

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In order to establish the expiration date of perishable food, growth curve data must be collected. Once the microbial load is so high that it poses a hazard to human health, the food item is no longer considered safe (expired). Generally a load of x50,000 bacteria/gram is considered unsafe. Your task is to determine the microbial growth curves for MicroYo, a new brand of yogurt. The growth is determined by sampling the yogurt and growing the bacterial isolates in broth culture which is then serially diluted by a total of x10,000 and inoculated onto standard petri plates of nutrient agar. The following colony counts are measured: Time (days) MicroYo colony count# 1 1 4 1 12 2 16 20 4 7 What day should you recommend expiring the yogurt (the last possible date before the microbial load is unsafe). 12 4 20 16

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Chapter 41, Problem 1COQ

Summary Introduction

To define: The terms negative feedback loop and positive feedback loop, and how they differ.

Introduction: In living organisms, maintenance of a steady condition is important to carry out the required metabolic and physiological processes. This steady state, called homeostasis, is maintained through a feedback system in the body. This feedback system is also used to maintain the conditions in the required range during any biological process.

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In order to establish the expiration date of perishable food, growth curve data must be collected. Once the microbial load is so high that it poses a hazard to human health, the food item is no longer considered safe (expired). Generally a load of x50,000 bacteria/gram is considered unsafe. Your task is to determine the microbial growth curves for MicroYo, a new brand of yogurt. The growth is determined by sampling the yogurt and growing the bacterial isolates in broth culture which is then serially diluted by a total of x10,000 and inoculated onto standard petri plates of nutrient agar. The following colony counts are measured: Time (days) MicroYo colony count# 1 1 4 1 12 2 16 20 4 7 What day should you recommend expiring the yogurt (the last possible date before the microbial load is unsafe). 12 4 20 16

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