Concept explainers
To compare:
The stains used for light microscope with those used for electron microscope.
Introduction:
Microscope is an instrument used to see objects that are not visible from the naked eyes. It was first used by scientist of Renaissance. There are three important parameters in microscopy which are magnification, resolution and contrast. Magnification is the enlargement of the object size. It is the ratio of object size to the real size. The clarity of the image is the measure of the resolution of the microscope whereas difference in the brightness of dark and light areas of the object is known as contrast parameter of the microscope.
Want to see the full answer?
Check out a sample textbook solutionChapter 4 Solutions
Campbell Biology in Focus; Modified Mastering Biology with Pearson eText -- ValuePack Access Card -- for Campbell Biology in Focus (2nd Edition)
- What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical dyes used to stain specimens for light microscopy?arrow_forwardWhat is the advantage of phase-contrast microscopyover bright-field microscopy?arrow_forwardWhat is phase contrast? Give examples of phase contrast in optical microscopy.arrow_forward
- What are the differences between phase contrast microscopy and differential interference microscopy?arrow_forwardDescribe the use of fluorescent dyes in microscopy.arrow_forwardExplain in detail simple and differential staining and also improving and adjusting contrast in light microscopy.arrow_forward
- What major advantage does phase-contrast microscopy haveover staining?arrow_forwardWhat's the difference between surface plasmon resonance spectroscopy and surface plasmon resonance microscopy?arrow_forwardExplain the differences between Brightfield, Darkfield, and Phase-Contrast Microscopy.arrow_forward
- Describe the process of Scanning Confocal Microscopy?arrow_forwardExplain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?arrow_forwarda) Briefly describe the concept of and instrument configuration for confocal microscopy. b) How do confocal and conventional microscopy compare? c) What other microscopy techniques can provide super-resolution?arrow_forward
- Principles Of Radiographic Imaging: An Art And A ...Health & NutritionISBN:9781337711067Author:Richard R. Carlton, Arlene M. Adler, Vesna BalacPublisher:Cengage Learning