Lab Manual for Biology
12th Edition
ISBN: 9781259298516
Author: Sylvia S. Mader Dr.
Publisher: McGraw-Hill Education
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Chapter 4, Problem 6A
Summary Introduction
Introduction:
Eukaryotes also possess plasma membrane like prokaryotes and it separates the content of cell from the environment and regulates the entry and exit of material in and out of cell. The presence of nucleus and internal membrane bound organelles distinguishes the eukaryotic cell from the prokaryotic cell.
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a) Find a nitrogen base. What carbon is the base attached to?
b) What two nitrogenous bases are held together by 2 hydrogen bonds?
c)What two nitrogenous bases are held together by 3 hydrogen bonds?
You will be building a double-stranded DNA molecule, representing a short (20 base) sequence that could be found in a chromosome. It will have a 3' --> 5' strand and a 5' --> 3' strand.
Use two full 12-base-pair DNA models.
a) Upload a photo of a model once the first 6 nucleotides have been inserted to base pair with the two parent strands.
b)Upload a photo of DNA polymerase I and Ligase in "action" removing the RNA primer nucleotides and making new phosphodiester bonds.
c)Upload a photo of the final two DNA strands, after Termination, identifying the parent and daughter strands of each.
Write down the 12 base-pairs (ex A-T or G-C) contained in the two models you will us from top to bottom below
Model 1 bp:
Model 2 bp:
Chapter 4 Solutions
Lab Manual for Biology
Ch. 4.1 - Explain why cells are alive but macromolecules are...Ch. 4.1 - Prob. 2CYPCh. 4.1 - Prob. 3CYPCh. 4.2 - 1. Explain the major differences between a...Ch. 4.2 - Prob. 2CYPCh. 4.3 - Summarize the benefits of compartmentalization...Ch. 4.3 - Prob. 2CYPCh. 4.3 - Prob. 3CYPCh. 4.4 - Prob. 1CYPCh. 4.4 - Explain the importance of the nuclear proes.
Ch. 4.4 - Prob. 3CYPCh. 4.5 - Prob. 1CYPCh. 4.5 - Prob. 2CYPCh. 4.5 - Prob. 3CYPCh. 4.6 - Prob. 1CYPCh. 4.6 - Prob. 2CYPCh. 4.7 - Prob. 1CYPCh. 4.7 - Prob. 2CYPCh. 4.7 - Explain why chloroplasts and mitochondria contain...Ch. 4.8 - Prob. 1CYPCh. 4.8 - Prob. 2CYPCh. 4.8 - Prob. 3CYPCh. 4 - Prob. 1ACh. 4 - The cell theory states that a. cells are the basic...Ch. 4 - Prob. 3ACh. 4 - Prob. 4ACh. 4 - Prob. 5ACh. 4 - Prob. 6ACh. 4 - Prob. 7ACh. 4 - Prob. 8ACh. 4 - The ______ is(are) responsible for protein...Ch. 4 - Prob. 10ACh. 4 - Prob. 11ACh. 4 - 12. Lysosomes function in
a. protein...Ch. 4 - Prob. 13ACh. 4 - Prob. 14ACh. 4 - Prob. 15ACh. 4 - Prob. 16ACh. 4 - Prob. 17ACh. 4 - Prob. 18ACh. 4 - Prob. 19ACh. 4 - Prob. 20ACh. 4 - Prob. 1TSCh. 4 - Prob. 2TSCh. 4 - Prob. 3TSCh. 4 - Prob. 4TS
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- a) What type of bond does helicase break? b) Which of these two types of bonds is stronger? c) Describe why it is necessary for the lagging strand to be made in fragments, called Okazaki fragments.arrow_forwarda) How are the model single nucleotides structurally different than real-life dNTPs? b)If you wanted to make these DNA nucleotides into RNA nucleotides, what could you do to them? c)Name the type of bond that is being formed by DNA Polymerase III.arrow_forwardYou will be building a double-stranded DNA molecule, representing a short (20 base) sequence that could be found in a chromosome. It will have a 3' --> 5' strand and a 5' --> 3' strand. Use two full 12-base-pair DNA models. a) Upload a picture of your model reflecting the Initiation phase of DNA replication. b) Upload a picture of your model once it has entered the Elongation phase of DNA replication, showing the action of Primase. c)Upload a picture of your model once it has entered the Elongation phase of DNA replication, showing the action of DNA Polymerase III.arrow_forward
- a)What region of the DNA do general transcription factors and RNA polymerase bind to? b)Where, in a eukaryotic cell, can one find TFs? c)What are the TFs called that bind to Enhancer Sites? d)Describe the role of Activators and Repressors in development.arrow_forwarda) Describe how mRNA and tRNA interact. b)Translate the following mRNA codons: AUG GUU AAC CAG UGA c) What are transcription factors made of?arrow_forwarda) State the Central Dogma of Molecular Biology in your own words. b) What enzyme synthesizes mRNA? c) Describe mRNA splicing.arrow_forward
- Give me detailed Solution. Don't give Ai generated solutionarrow_forwarda) The lux operon is under positive control. Based on this information, does the luxR regulator sequence make a repressor protein or an activator protein? b) How will binding of this complex affect RNA polymerase? Remember this operon is under positive control. c) AHL is a signal molecule that V. fisheri makes to communicate with neighboring bacterial cells. This molecule can diffuse outside of the cell and into another bacterial cell in close proximity. This type of communication between bacterial cells is known as quorum sensing. If bacterial cell density is low how will this affect the lux operon? What will happen if the density is high?arrow_forward1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’ “met-_________-_________-_________-_________-_________”STOP” 2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results. Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn A. Missense B. Silent C. Nonsense D. Frameshift ______ Pro-Thr-Ser-STOP ______ Pro-Thr-Ser-Leu-Ile-His-Asn ______ Pro-Thr-Ser-Leu-Leu-His-Asn _______ Pro-Thr-His-Cys-Tyr-Thrarrow_forward
- Referring to the Standard Genetic Code table, categorize the chemical properties of each of the 24 amino acids that make up the ER Signal Peptide (hydrophobic, hydrophilic, positive charge, or negative charge). What is notable about the chemical properties of the amino acids that make up the ER Signal Peptide? methionine- translation code tra-hyperopic amino acid R Origine eukiauk aicd amino glutamine HYDOICO ACIDS gaac -CHANGED AMINO ACIDarrow_forward1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’ “met-_________-_________-_________-_________-_________”STOP” 2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results. Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn A. Missense B. Silent C. Nonsense D. Frameshift ______ Pro-Thr-Ser-STOP ______ Pro-Thr-Ser-Leu-Ile-His-Asn ______ Pro-Thr-Ser-Leu-Leu-His-Asn _______ Pro-Thr-His-Cys-Tyr-Thrarrow_forwarda) The relationship between the Hawaiian bobtail squid and V. fischeri is symbiotic where both species benefit. What is the benefit to each? b) Why might quorum sensing be beneficial to pathogenic bacteria? c) How might scientists use quorum sensing to treat bacterial infections?arrow_forward
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