A reasonable explanation for the relation between % of GC base pairs in DNA and the melting temperature needs to be given. Concept introduction: The GC base pair i.e. Guanine - Cytosine pair help the DNA to maintain its helical structure which is dependent on the nucleotide sequences. A base pair is a unit structure which contains two nucleotides connected by hydrogen bonds to each other. These nucleotide strand connected by the hydrogen bond of the base pairs are stable at room temperature, but they separate above a melting point which is defined by the length of the molecule, the extent of mispairing and the amount of GC content in it. Higher GC content makes the melting point higher.

Question

Want to see the full answer?

Answer 1

Question

Chapter 4, Problem 50P

Interpretation Introduction

Interpretation:

A reasonable explanation for the relation between % of GC base pairs in DNA and the melting temperature needs to be given.

Concept introduction:

The GC base pair i.e. Guanine - Cytosine pair help the DNA to maintain its helical structure which is dependent on the nucleotide sequences. A base pair is a unit structure which contains two nucleotides connected by hydrogen bonds to each other. These nucleotide strand connected by the hydrogen bond of the base pairs are stable at room temperature, but they separate above a melting point which is defined by the length of the molecule, the extent of mispairing and the amount of GC content in it. Higher GC content makes the melting point higher.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

The enzyme lactate dehydrogenase (LDH) catalyzes the conversion of pyruvate to lactatein skeletal muscle cells using NAD/NADH during anaerobic “balanced” fermentation.Answer the following questions about this reaction. (a) Write out the two reductive half reactions and indicate the E ̊' for each half reaction. Write out the full balanced reaction for the pyruvate to lactate rxn and indicate the ∆E ̊' for the reaction. (b) What is the free energy change under standard state conditions for thisreaction? Which direction is spontaneous?(c) Assume that in skeletal muscle cells the ratio of [NAD+] to [NADH] is 100, and that the[pyruvate] = 0.40 mM and [lactate] = 4.0 mM. What is the free energy change (∆G')for the conversion of pyruvate to lactate? Indicate the direction in which the reactionis spontaneous under these cellular conditions.

Why did the authors worry about the temperature-dependent solubility of the carriers in thebilayer? How did the authors determine whether the effect of freezing the lipid bilayer wasto decrease the solubility of the carriers (nonactin and valinomycin) or whether the effectwas to impair their ability to diffuse through the membrane (decrease their mobility)?

Kranse et. al. measured the temperature dependence of conductance using membranescontaining the phospholipids glyceryl dipalmitate and glyceryl distearate. Describe themodifications in membrane content that you would employ to: (a) shift the temperature of the phase transition (b) make the ion conductance curve for valinomycin andnonactin more like that of gramicidin