EBK PRINCIPLES OF BIOLOGY
EBK PRINCIPLES OF BIOLOGY
2nd Edition
ISBN: 8220103675338
Author: BROOKER
Publisher: YUZU
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Chapter 4, Problem 2TY
Summary Introduction

Introduction:

The technique of microscopy helps to identify micro-organisms and visualize various physiological features of the organism under study. Different types of microscopes are used for various purposes that allow easy detection and exhaustive study of the structure and properties of microbes.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.

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EBK PRINCIPLES OF BIOLOGY

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