FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
5th Edition
ISBN: 9781119661450
Author: Voet
Publisher: WILEY
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Chapter 4, Problem 25CQ
Summary Introduction

To draw: The structure of ATLDAK.

Concept introduction: Each amino acid has unique ionization characteristics that vary at different pH. Ionizable groups such as amino group, carboxylic group at certain pH remain protonated and when the pH increases, it becomes deprotonated. The isoelectric point is the pH at which net electric charge of a molecule is zero.

(a)

Summary Introduction

To determine: The isoelectric point (pI) of ATLDAK.

Concept introduction: Each amino acid has unique ionization characteristics that vary at different pH. Ionizable groups such as amino group, carboxylic group at certain pH remain protonated and when the pH increases, it becomes deprotonated. The isoelectric point is the pH at which net electric charge of a molecule is zero.

(b)

Summary Introduction

To determine: The net charge of ATLDAK at pH 7.0.

Concept introduction: Each amino acid has unique ionization characteristics that vary at different pH. Ionizable groups such as amino group, carboxylic group at certain pH remain protonated and when the pH increases, it becomes deprotonated. The isoelectric point is the pH at which net electric charge of a molecule is zero.

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Please help with this Mass Spectrometry Question. Thank you For the mass spec. shown in the attached image, please determine and give the amino acid sequence of the pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. How does one arrive at the solution?
Draw a tripeptide of your choosing at pH 7. Have the N-terminus on the left and the C-terminus on the right. Then: Draw a triangle around the α-carbons. Draw a box around the R-groups. Circle the atoms capable of hydrogen bonding. Highlight the atoms involved in the formation of the peptide bonds. What type of structure have you drawn? (primary, secondary, tertiary or quaternary protein structure).
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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY