Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Chapter 4, Problem 2.3SC
Summary Introduction
To determine: The reason why the colored photos of electron micrographs are called as false color or colorized.
Introduction: An electron microscope (EM) helps to visualize very small structures that cannot be viewed under a light microscope. There are two types of EM namely, transmission electron microscope (TEM) and scanning electron microscope (SEM). The photographs taken on an electron microscope is termed as electron micrographs.
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Chapter 4 Solutions
Microbiology: Principles and Explorations
Ch. 4 - Prob. 1.1SCCh. 4 - Prob. 1.2SCCh. 4 - Why would radio waves and microwaves be unsuitable...Ch. 4 - Would standard immersion oil placed on a plastic...Ch. 4 - Prob. 2.1SCCh. 4 - How can you distinguish between TEM and SEM...Ch. 4 - Prob. 2.3SCCh. 4 - Prob. 2.4SCCh. 4 - What can be observed in a wet-mount or...Ch. 4 - What is the difference between simple stains and...
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- Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.arrow_forwardWhy is it useful to stain chromosomes?arrow_forwardWhich of the following technique is FALSE in microscopy? Osmium tetroxide can be used as a fixative as well as a negative stain in transmission electron microscopy. Two proteins; each tagged with their individual primary antibody followed by one protein with rhodamine conjugated secondary antibody and the other with fluorescein-conjugated secondary antibody can be visualized simultaneously using a fluorescence microscope. The emission spectrum of Rhodamine is 580 nm The emission spectrum of Fluorescein is 521 nm O An objective lens with 100X magnification and projection lens with 10X magnification is going to produce a 1000X total magnification. Phase contrast and differential interference contrast (DIC) do not require staining.arrow_forward
- In lab, we used the Gram stain to differentiate between Gram-positive and Gram-negative bacteria. Some bacteria stain purple (retaining the crystal violet) while others stain pink (from the counter-stain safranin). a) What is the biological molecule that is present in these bacteria that produces different results between Gram-positive and Gram-negative bacteria (be specific!) AND b) What is the difference between Gram-positive and Gram-negative bacteria that creates the different staining patterns (purple vs. pink)? (2-3 sentences max)arrow_forwardWhy does the nucleus appear deep blue after staining with methylene blue? Is it possible that bacterial cells are also present in the preparation?arrow_forwardWhy is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.arrow_forward
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