BIOLOGY
BIOLOGY
14th Edition
ISBN: 9781265624033
Author: Mader
Publisher: MCG
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Chapter 4, Problem 1TC
Summary Introduction

To conclude:

Regarding the origin of the plastids.

Introduction:

Plastid is a major “double membrane organelle. It is found in cells of algae, plants and few eukaryotic cells. They are the site for manufacturing and storage of chemical compounds that are important for the functioning of the cells.

Expert Solution & Answer
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Explanation of Solution

Protists that belong to phylum Apicomplexa causes malaria and contribute to infections associated with acquired immunodeficiency syndrome (AIDS). These parasites contain plastids and chloroplast is a type of plastid. The plastid and the enzymes associated with it are necessary for the survival, thus inhibition of the enzymes is lethal.

The antibiotic that inhibits the functioning of prokaryotic enzymes kills the parasite by “acting against the plastids of the parasite”. This explains the prokaryotic origin of the plastid and it’s DNA. The DNA encodes for the prokaryotic proteins (enzymes) due to this its enzyme can by inhibited by using antibiotics which are used against the enzymes of prokaryotes.

Thus, the parasite probably obtained the plastids by endosymbiosis in the same way that eukaryotic cell have acquired chloroplast and mitochondria.

Conclusion

The origin of the plastids is probably by endosymbiosis.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
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