CUSTOM PRESCOTT'S MICROBIOLOGY
CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 39.1, Problem 3MI
Summary Introduction

Pathogenic streptococci are characterized by a heterogeneous group of Gram-positive bacteria. One of the most important bacterial pathogens in this group is Streptococcus pyogenes. Streptococcus pyogenes belongs to group A, β-hemolytic streptococci that play a major role in developing both invasive and non-invasive infections in humans. The pathogenic group A streptococci (GAS) secretes various exotoxins, which play a crucial role in developing bacterial virulence. The virulence factor enables the bacteria to attach to the host cells, evade the immune response, and thereby spreads the host cells by penetration.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin

Chapter 39 Solutions

CUSTOM PRESCOTT'S MICROBIOLOGY

Ch. 39.1 - What is the mechanism by which PTx kills host...Ch. 39.1 - Prob. 9CCCh. 39.1 - Prob. 10CCCh. 39.2 - What are the three stages of Lyme disease? What...Ch. 39.2 - Prob. 1CCCh. 39.2 - Prob. 2CCCh. 39.2 - Prob. 3CCCh. 39.2 - Prob. 4CCCh. 39.3 - What are the chief differences between tuberculoid...Ch. 39.3 - How does H. pylori increase the local pH in its...Ch. 39.3 - Prob. 3MICh. 39.3 - Prob. 4MICh. 39.3 - Prob. 5MICh. 39.3 - Prob. 1CCCh. 39.3 - Why do you think the slow growth rate of M. leprae...Ch. 39.3 - Prob. 3CCCh. 39.3 - Prob. 4CCCh. 39.3 - Prob. 5CCCh. 39.3 - Prob. 6CCCh. 39.3 - Prob. 7CCCh. 39.3 - What is the difference between S. aureus and MRSA?Ch. 39.3 - Prob. 9CCCh. 39.3 - Prob. 10CCCh. 39.3 - Prob. 11CCCh. 39.4 - How do the neurological effects of botulinum toxin...Ch. 39.4 - To which E. coli class does the strain 0157:H7...Ch. 39.4 - Prob. 1CCCh. 39.4 - Compare the gastroenteritis caused by Vibrio...Ch. 39.4 - Prob. 3CCCh. 39.4 - Prob. 4CCCh. 39.4 - Prob. 5CCCh. 39.4 - Prob. 6CCCh. 39.4 - Prob. 7CCCh. 39.5 - Prob. 1MICh. 39.5 - Prob. 1CCCh. 39.5 - Prob. 2CCCh. 39.5 - How is ornithosis transmitted?Ch. 39.5 - Prob. 4CCCh. 39.6 - What cells and tissues make up the pseudomembrane?Ch. 39.6 - Prob. 2MICh. 39.6 - In both C. difficileassociated disease and...Ch. 39.6 - Prob. 2CCCh. 39.6 - Prob. 3CCCh. 39.6 - Prob. 4CCCh. 39 - Prob. 1RCCh. 39 - Prob. 2RCCh. 39 - Prob. 3RCCh. 39 - Prob. 4RCCh. 39 - Prob. 5RCCh. 39 - Prob. 1ALCh. 39 - Prob. 2ALCh. 39 - Prob. 3ALCh. 39 - Prob. 4ALCh. 39 - Prob. 5ALCh. 39 - Prob. 6AL
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