IA MODIFIED MASTERING BIOLOGY WITH E TEX
12th Edition
ISBN: 9780136781752
Author: Urry
Publisher: PEARSON
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Chapter 39.1, Problem 1CC
Summary Introduction
To know: The differences between the dark and light-grown plants and the role of etiolation in a seedling competing successfully.
Introduction: Etiolation can be defined as the process by which plants are grown without sunlight. The plants and seedlings which are grown in absence of light in dark are known as etiolated plants or etiolated seedlings.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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1)What is the selectable marker in this experiment? How…
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In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
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Chapter 39 Solutions
IA MODIFIED MASTERING BIOLOGY WITH E TEX
Ch. 39.1 - Prob. 1CCCh. 39.1 - Cycloheximide is a drug that inhibits protein...Ch. 39.1 - Prob. 3CCCh. 39.2 - Prob. 1CCCh. 39.2 - WHAT IF? If a plant has the double mutation err...Ch. 39.2 - MAKE CONNECTIONS What type of feedback process is...Ch. 39.3 - If an enzyme in field-grown soybean leaves is most...Ch. 39.3 - WHAT IF? If a plant flowers in a controlled...Ch. 39.3 - Prob. 3CCCh. 39.4 - Prob. 1CC
Ch. 39.4 - Prob. 2CCCh. 39.4 - Prob. 3CCCh. 39.5 - Prob. 1CCCh. 39.5 - Chewing insects mechanically damage plants and...Ch. 39.5 - Many fungal pathogens get food by causing plant...Ch. 39.5 - Prob. 4CCCh. 39 - What are two common ways by which signal...Ch. 39 - Is there any truth to the old adage, "One bad...Ch. 39 - Why did plant physiologists propose the existence...Ch. 39 - Plants that have acclimated to drought stress are...Ch. 39 - Prob. 39.5CRCh. 39 - Prob. 1TYUCh. 39 - Prob. 2TYUCh. 39 - Prob. 3TYUCh. 39 - How may a plant respond to severe heat stress? (A)...Ch. 39 - Level 2: Application/Analysis 5. The signaling...Ch. 39 - If a long-day plant has a critical night length of...Ch. 39 - Prob. 7TYUCh. 39 - DRAW IT Indicate the response to each condition by...Ch. 39 - Level 3: Synthesis/Evaluation 9. EVOLUTION...Ch. 39 - SCIENTIFIC INQUIRY A plant biologist observed a...Ch. 39 - SCIENCE. TECHNOLOGY, AND SOCIETY Describe how our...Ch. 39 - Prob. 12TYUCh. 39 - Prob. 13TYU
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