PRESCOTT'S MICROBILOGY
11th Edition
ISBN: 9781264075515
Author: WILLEY
Publisher: MCG
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Chapter 38.3, Problem 2CC
Summary Introduction
To determine: The reason for recurrent cold sores in people.
Introduction: Cold sore or a fever blister is a fluid-filled lesion that is commonly formed at the lips or the inside of nostrils. Cold sores present themselves near the nerve endings. As a result, they cause significant itching and burning sensation.
Summary Introduction
To determine: The causative agents of cold sores.
Introduction: Viruses consists of
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 38 Solutions
PRESCOTT'S MICROBILOGY
Ch. 38.1 - Prob. 1MICh. 38.1 - Why is it thought that the characteristic rash is...Ch. 38.1 - Prob. 3MICh. 38.1 - Prob. 1CCCh. 38.1 - Prob. 2CCCh. 38.1 - Prob. 3CCCh. 38.1 - Prob. 4CCCh. 38.1 - Prob. 5CCCh. 38.1 - Prob. 6CCCh. 38.1 - Prob. 7CC
Ch. 38.3 - Prob. 1MICh. 38.3 - Prob. 2MICh. 38.3 - Prob. 1CCCh. 38.3 - Prob. 2CCCh. 38.3 - Prob. 3CCCh. 38.3 - Prob. 4CCCh. 38.3 - Prob. 5CCCh. 38.3 - Prob. 6CCCh. 38.4 - Prob. 1CCCh. 38.4 - Prob. 2CCCh. 38.4 - Prob. 3CCCh. 38.4 - Prob. 4CCCh. 38.5 - Prob. 1MICh. 38.5 - Prob. 1CCCh. 38.5 - Prob. 2CCCh. 38.5 - Prob. 3CCCh. 38.6 - How are prions different from viruses? How are...Ch. 38.6 - In what way are spongiform encephalopathies...Ch. 38 - Prob. 1RCCh. 38 - Prob. 2RCCh. 38 - Prob. 3RCCh. 38 - Prob. 1ALCh. 38 - Prob. 2ALCh. 38 - Prob. 3ALCh. 38 - Prob. 4ALCh. 38 - Prob. 5ALCh. 38 - Prob. 6AL
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
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