Biology: Concepts and Applications (MindTap Course List)
Biology: Concepts and Applications (MindTap Course List)
9th Edition
ISBN: 9781285427812
Author: Cecie Starr, Christine Evers, Lisa Starr
Publisher: Cengage Learning
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Chapter 38, Problem 6SQ
Summary Introduction

Introduction:

There are a number of animals present in the environment. Animals can live in a variety of habitats. All the animals have the capability to produce their offspring by the process of reproduction. The offspring matures and reproduces to form the next generation of animals. Reproduction is a very important process as it maintains the existence of an organism in the world.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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