Biology
12th Edition
ISBN: 9780134813448
Author: Audesirk, Teresa, Gerald, Byers, Bruce E.
Publisher: Pearson,
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Chapter 37.1, Problem 1CYL
compare and contrast the terms “microbe” and “pathogen”?
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The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
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Mutation 1
Mutation 2
There is currently a H5N1 cattle outbreak in North America.
According to the CDC on Feb 26*: "A multistate outbreak of HPAI A(H5N1) bird flu in dairy cows was first reported on March 25, 2024. This is the first time that these bird flu viruses had been found in cows. In the United States, since 2022, USDA has reported HPAI A(H5N1) virus detections in more than 200 mammals."
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You have a bacterial strain with the CMU operon:
a) As shown in the image below, the cmu operon encodes a peptide (Pep1), as well as a
kinase and regulator corresponding to a two-component system. The cmu operon is
activated when Pep 1 is added to the growth media.
Pep1 is a peptide that when added extracellularly leads to activation of the Cmu operon.
Pep1 cmu-kinase cmu-regulator
You also have these genetic components in other strains:
b) An alternative sigma factor, with a promoter activated by the cmu-regulator, that control a
series of multiple operons that together encode a transformasome (cellular machinery
for transformation).
c) the gene cl (a repressor).
d) the promoter X, which includes a cl binding site (and in the absence of cl is active).
e) the gene gp (encoding a green fluorescence protein).
Using the cmu operon as a starting point, and assuming you can perform cloning to rearrange
any of these genomic features, how would you use one or more of these to modify the…
Chapter 37 Solutions
Biology
Ch. 37.1 - compare and contrast the terms microbe and...Ch. 37.1 - Prob. 2CYLCh. 37.2 - Prob. 1TCCh. 37.2 - If phagocytes kill most species of bacteria that...Ch. 37.2 - describe the external barriers to Infection,...Ch. 37.2 - Prob. 2CYLCh. 37.3 - Prob. 1CYLCh. 37.3 - Prob. 2CYLCh. 37.4 - Prob. 1TCCh. 37.4 - Prob. 1CYL
Ch. 37.4 - Prob. 2CYLCh. 37.4 - Prob. 3CYLCh. 37.5 - Prob. 1CYLCh. 37.5 - Prob. 2CYLCh. 37.6 - Prob. 1CYLCh. 37.6 - Prob. 2CYLCh. 37.7 - Prob. 1HYEWCh. 37.7 - Prob. 1TCCh. 37.7 - explain how antibiotics, antiviral drugs, and...Ch. 37.7 - Prob. 2CYLCh. 37.8 - What might be the evolutionary advantage of...Ch. 37.8 - Prob. 1CYLCh. 37.9 - Prob. 1CYLCh. 37.9 - describe current medical treatments against...Ch. 37.9 - Prob. 1CTCh. 37 - Prob. 1MCCh. 37 - Prob. 2MCCh. 37 - Molecules that label your cells as self are a....Ch. 37 - Prob. 4MCCh. 37 - The immune system usually does not attack your...Ch. 37 - External defenses against microbial invasion...Ch. 37 - Prob. 2FIBCh. 37 - Prob. 3FIBCh. 37 - Prob. 4FIBCh. 37 - Prob. 5FIBCh. 37 - Prob. 6FIBCh. 37 - Prob. 1RQCh. 37 - Prob. 2RQCh. 37 - Describe humoral immunity and cell-mediated...Ch. 37 - Prob. 4RQCh. 37 - Prob. 5RQCh. 37 - Prob. 6RQCh. 37 - Prob. 7RQCh. 37 - What are memory cells? How do they contribute to...Ch. 37 - Prob. 9RQCh. 37 - Prob. 10RQCh. 37 - Prob. 11RQCh. 37 - Prob. 12RQCh. 37 - Prob. 13RQCh. 37 - Why is it essential that antibodies and T-cell...Ch. 37 - Prob. 2AC
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- You have identified a new species of a Gram-positive bacteria. You would like to screen their genome for all proteins that are covalently linked to the cell wall. You have annotated the genome, so that you identified all the promoters, operons, and genes sequences within the operons. Using these features, what would you screen for to identify a set of candidates for proteins covalently linked to the bacterial cell wall.arrow_forwardBelow is a diagram from a genomic locus of a bacterial genome. Each arrow represents a coding region, and the arrowheads indicate its orientation in the genome. The numbers are randomly assigned. Draw the following features on the diagram, and explain your rationale for each feature: 10 12 合會會會會長 6 a) Expected transcriptions, based on known properties of bacterial genes and operons. How many proteins are encoded in each of the transcripts? b) Location of promoters (include rationale) c) Location of transcriptional terminators (include rationale) d) Locations of Shine-Dalgarno sequences (include rationale)arrow_forwardSample excuse letter in school class for the reasons of headaches and dysmenorrhea caused by menstrual cyclearrow_forward
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