BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
BIOLOGY(LL)-W/ACCESS CODE >CUSTOM<
12th Edition
ISBN: 9781264058167
Author: Raven
Publisher: MCG CUSTOM
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Chapter 37, Problem 2U
Summary Introduction

Introduction:

The soil is formed by the weathering of rocks. It is present in the form of different layers on the surface of the crust of the Earth. The soil consists of sand, silt, clay, minerals, organic matter, humus and air in the pore space.

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a) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following:  Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng  Final recovery volume: 0.50 mL  Volume plated: 0.25 mL  Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) b) Using the equation above, calculate the transformation efficiency. c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?   b) Predict the growth you would expect to see on each of the following plates:         – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?  2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following:  Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng  Final recovery volume: 0.50 mL  Volume plated: 0.25 mL  Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
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