Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 37, Problem 1CA
Bacteriophages are being explored as a treatment for antibiotic-resistant bacteria. Among the advantages of bacteriophages is that they are self-replicating and self-limiting. They only infect bacteria so are not toxic to humans. Explain how this is different from antibiotic therapy.
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You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body.
You finally find a phage that you think will work because it attaches to and injects its genome into the patient's Mycobacterium isolate. Next, you need to be sure of what characteristics of the phage infection cycle? Pick all that are true
A. That the phage delivers new antibiotic resistance genes to Mycobacterium via transduction
B. That the correct proteins are expressed during the phages metabolism
C. That the infection is lytic
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You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body.
You need to find a phage that infects the Mycobacterium you isolated from the patient.
Which TWO options have the highest chance of success?
Group of answer choices
1.Isolating new phage from soil using a plaque assay with the Mycobacterium isolate
2.Choosing likely candidates from analysis of phage 16S rRNA phylogeny in the tree of life
3.Obtaining a very well-studied phage, such as T4 bacteriophage, that specifically targets E. coli
4.Screening a colleague's library of known Mycobacterium phages for phage that…
(there are multiple right answers to this homework question but I can't tell which one is right)
You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body.
In the first step of your phage therapy design process, you isolate the exact Mycobacterium strain from the patient. What is the most important thing must you establish next about this bacterial isolate?
That the isolate is genomically stable so you can study it
That the isolate is similar to Mycobacterium in other patients
That the isolate rapidly evolves due to horizontal gene transfer
That the Mycobacterium is…
Chapter 37 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 37 - Why did you add Escherichia coli to sewage, which...Ch. 37 - If there were no plaques on your plates, offer an...Ch. 37 - How would you develop a pure culture of a phage?Ch. 37 - How would you isolate a bacteriophage for a...Ch. 37 - Bacteriophages are being explored as a treatment...Ch. 37 - Rotavirus grows in cells at the tips of intestinal...
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- The five stages of the bacteriophage lytic cycle occur in this order:a. penetration, attachment, release, maturation, biosynthesis.b. attachment, penetration, release, biosynthesis, maturation.c. biosynthesis, attachment, penetration, maturation, release.d. attachment, penetration, biosynthesis, maturation, release.e. penetration, biosynthesis, attachment, maturation, release.arrow_forwardA patient is asked to participate in a trial using a bacteriophage to treat a Streptococcus infection. The patient is worried that she will end up with a viral infection rather than a Streptococcal infection. How would you address her concerns? – discuss the specificity of viral infections. How do they infect host cells, is that a random process?arrow_forwardPenicillin was first used in the 1940s to treat gonorrhea infections produced by the bacterium Neisseria gonorrhoeae. In 1984, according to the CDC, fewer than 1% of gonorrhea infections were caused by penicillin-resistant N. gonorrhoeae. By 1990, more than 10% of cases were penicillin resistant and a few years later the level of resistance was 95%. Explain the various ways this resistance could be spread among the cells. Could this resistance pass to other infectious bacteria from N. gonorrhoeae?arrow_forward
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