Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
3rd Edition
ISBN: 9780134895727
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky
Publisher: PEARSON
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Textbook Question
Chapter 34.7, Problem 2CC
How does the Bohr shift help deliver O2, to very active tissues?
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 34 Solutions
Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
Ch. 34.1 - How is the now of hemolymph through an open...Ch. 34.1 - Three-chambered hearts with incomplete septa were...Ch. 34.1 - Prob. 3CCCh. 34.2 - Prob. 1CCCh. 34.2 - Why is it important that the AV node delay the...Ch. 34.2 - Prob. 3CCCh. 34.3 - What is the primary cause of the low velocity of...Ch. 34.3 - Prob. 2CCCh. 34.3 - Prob. 3CCCh. 34.4 - Prob. 1CC
Ch. 34.4 - Prob. 2CCCh. 34.4 - Prob. 3CCCh. 34.4 - Prob. 4CCCh. 34.5 - Why is an internal location for gas exchange...Ch. 34.5 - After a heavy rain, earthworms come to the...Ch. 34.5 - MAKE CONNECTIONS Describe similarities in the...Ch. 34.6 - Prob. 1CCCh. 34.6 - Prob. 2CCCh. 34.6 - WHAT IF? If an injury tore a small hole in the...Ch. 34.7 - What determines whether O2, or CO2, undergoes net...Ch. 34.7 - How does the Bohr shift help deliver O2, to very...Ch. 34.7 - Prob. 3CCCh. 34 - Which of the following respiratory systems is not...Ch. 34 - Blood returning to the mammalian heart in a...Ch. 34 - Pulse is a direct measure of A. blood pressure. B....Ch. 34 - Prob. 4TYUCh. 34 - One feature that amphibians and humans have in...Ch. 34 - If a molecule of CO2 released into the blood in...Ch. 34 - Prob. 7TYUCh. 34 - DRAW IT Plot blood pressure against time for one...Ch. 34 - Prob. 9TYUCh. 34 - Prob. 10TYUCh. 34 - Prob. 11TYUCh. 34 - SYNTHESIZE YOUR KNOWLEDGE The diving bell spider...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
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