Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
3rd Edition
ISBN: 9780134895727
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky
Publisher: PEARSON
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Chapter 33.2, Problem 1CC
Summary Introduction
To distinguish:
The overall structure of a gastrovascular cavity from that of an alimentary canal.
Introduction:
Digestion is the process of conversion of larger molecules into smaller and simpler molecules which can be used the organism for the generation of ATP molecules. The processing of food in special compartments eliminates the risk of self digestion. Many animals have simpler body plan while other have complex body plans, so the digestion takes place accordingly in the animal.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 33 Solutions
Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
Ch. 33.1 - An animal requires 20 amino acids to make...Ch. 33.1 - Prob. 2CCCh. 33.1 - WHAT IF? if a zoo animal eating ample food shows...Ch. 33.2 - Prob. 1CCCh. 33.2 - In what sense are nutrients from a recently...Ch. 33.2 - Prob. 3CCCh. 33.3 - How does swallowed food reach the stomach of a...Ch. 33.3 - Explain why a proton pump inhibitor, such as the...Ch. 33.3 - Prob. 3CCCh. 33.4 - Prob. 1CC
Ch. 33.4 - Prob. 2CCCh. 33.4 - Prob. 3CCCh. 33.5 - Prob. 1CCCh. 33.5 - The energy required to maintain each gram of body...Ch. 33.5 - Prob. 3CCCh. 33 - The mammalian trachea and esophagus both connect...Ch. 33 - Which organ is incorrectly paired with its...Ch. 33 - Which of the following is not a major activity of...Ch. 33 - Prob. 4TYUCh. 33 - Prob. 5TYUCh. 33 - If you were to jog 1 km a few hours after lunch,...Ch. 33 - Prob. 7TYUCh. 33 - FOCUS ON EVOLUTION The human esophagus and trachea...Ch. 33 - Prob. 9TYUCh. 33 - Prob. 10TYU
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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