Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Chapter 33, Problem 33.5QAP
Give the advantages and disadvantages of sequential injection analyzers compared to traditional flow Injection analyzers.
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The amount of caffeine in an analgesic tablet was determined by HPLC using a
normal calibration curve. Standard solutions of caffeine were prepared and analyzed
using a 10-µL fixed-volume injection loop. Results for the standards are summarized
in the following table.
Concentration of Standards
Signal
(arbitrary units)
(ppm)
50.0
8354
100.0
16925
150.0
25218
200.0
33584
250.0
42002
The sample was prepared by placing a single analgesic tablet in a small beaker and
adding 10 mL of methanol. After allowing the sample to dissolve, the contents of the
beaker, including the insoluble binder, were quantitatively transferred to a 25-mL
volumetric flask and diluted to volume with methanol. The sample was then filtered,
and a 1.00-mL aliquot was transferred to a 10-mL volumetric flask and diluted to
volume with methanol. When analyzed by HPLC, the signal for the caffeine was
found to be 21469. Report the number of milligrams of caffeine in the analgesic
tablet.
analyte
concentration(C)(mg/ml)
injection volume (ul)
elution time (time)
peak DAD signal(mAU)
caffeine
1
1
4.67
302.85
aspartame
5
1
7.53
15.83
benzoic acid
1
1
8.14
89.98
saccharin
1
1
1.91
84.86
mixture(add everything above with 1:1:1:1 ratio)
1
4.47
69.58
How to get the concentration of the mixture in this case?
What are pipeline slurry systems? How do they function?
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