BIOCHEMISTRY (LOOSELEAF)-W/ACCESS
BIOCHEMISTRY (LOOSELEAF)-W/ACCESS
9th Edition
ISBN: 9781319425784
Author: BERG
Publisher: Macmillan Higher Education
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Chapter 33, Problem 13P
Interpretation Introduction

Interpretation:

The mechanism of acetylation of lysine residue from acetyl-CoA must be determined.

Concept introduction:

Acetylation is process in which acetyl functional group is introduced into a chemical compound. Histone proteins are basically acetylated for loosening of binding between DNA and histone. This helps the RNA pol and other factors to bind to DNA and prepare it for gene expression.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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