EP CAMPBELL BIO.IN FOCUS AP-MOD.MASTER.
EP CAMPBELL BIO.IN FOCUS AP-MOD.MASTER.
3rd Edition
ISBN: 9780137453092
Author: Urry
Publisher: SAVVAS L
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Chapter 31.2, Problem 1CC

If an enzyme in field-grown soybean leaves is most active at noon and least active at midnight, is its activity under circadian regulation?

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Explain how coumarins are usually stored in the vegetable in an un-elicited tissue, compared to a vegetable under conditions of biotic (or abiotic) stress in which, for example, leaf tissue disruption occurs. What is the relationship of this condition to the regulation of coumarin biosynthesis? Describe how compartmentalization / accumulation of this metabolite occurs in the cell and point out a biosynthetic step in which key enzymes are involved in this condition.
J. C. Servaites, in Plant Physiol. (1985) 78:839–843, observed that Rubisco from tobacco leaves collected before dawn had a much lower specific activity than the enzyme collected at noon. This difference persisted despite extensive dialysis, gel filtration, or heat treatment. However, precipitation of the predawn enzyme by 50% (NH4)2SO4 restored the specific activity to the level of the noon-collected enzyme. Suggest an explanation.
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