Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
3rd Edition
ISBN: 9780134895727
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky
Publisher: PEARSON
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Textbook Question
Chapter 31.1, Problem 2CC
WHAT IF? If a plant has the double mutation ctr and ein, what is its triple-response
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In roses, the synthesis of red pigment is produced by two steps in a pathway.
gene O
magenta intermediate -
gene P
colorless intermediate-
red pigment
What would the phenotype be of a plant homozygous for a null mutation of gene P?
What would the phenotype be of a plant homozygous for a null mutation of gene Q?
What would the phenotype be of a plant homozygous for null mutations of genes P and Q?
magenta
red
Match a genotype to each strain.
colorless
Strain
P locus Q locus
homozygous null mutation of gene P
homozygous null mutation of gene Q
homozygous null mutations of genes P and Q
Answer Bank
plp
PIP
What F2 ratio is expected from crossing a plant that is homozygous for a null mutation of gene P with a plant that is
homozygous for a null mutation of gene Q? Assume independent assortment.
9 colorless : 4 magenta : 3 red
9 red : 4 colorless : 3 magenta
O 9 red : 4 magenta : 3 colorless
A. The genes CLV3, CLV1/2 and WUS are involved in maintaining the size of shoot apical meristem. Diagram the interactions among these gene products, using ‘—>’ for positive interactions (i.e. activation) and ‘ —|’ for negative interactions (i.e. repression).
B. What phenotype would you expect for a double mutant clv3 wus? And why?
In roses, the synthesis of red pigment is by two steps ina pathway, as follows:colorless intermediate gene Pmagenta intermediate red pigment gene Qa. What would the phenotype be of a plant homozygousfor a null mutation of gene P?b. What would the phenotype be of a plant homozygousfor a null mutation of gene Q?c. What would the phenotype be of a plant homozygousfor null mutations of genes P and Q?d. Write the genotypes of the three strains in parts a, b,and c.e. What F2 ratio is expected from crossing plants fromparts a and b? (Assume independent assortment.)
Chapter 31 Solutions
Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
Ch. 31.1 - Prob. 1CCCh. 31.1 - WHAT IF? If a plant has the double mutation ctr...Ch. 31.1 - Prob. 3CCCh. 31.2 - If an enzyme in field-grown soybean leaves is most...Ch. 31.2 - WHAT IF? If a plant flowers in a controlled...Ch. 31.2 - MAKE CONNECTIONS Plants detect the quality of...Ch. 31.3 - Prob. 1CCCh. 31.3 - Prob. 2CCCh. 31.3 - Prob. 3CCCh. 31.4 - Prob. 1CC
Ch. 31.4 - Chewing insects mechanically damage plants and...Ch. 31.4 - Prob. 3CCCh. 31 - The hormone that helps plants respond to drought...Ch. 31 - Prob. 2TYUCh. 31 - Prob. 3TYUCh. 31 - Prob. 4TYUCh. 31 - Prob. 5TYUCh. 31 - Prob. 6TYUCh. 31 - DRAW IT Indicate the response of Arabidopsis to...Ch. 31 - Prob. 8TYUCh. 31 - FOCUS ON EVOLUTION As a general rule,...Ch. 31 - FOCUS ON INTERACTIONS In a short essay (100-150...Ch. 31 - Prob. 11TYU
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- please explain if it would be elongated or shortened expression! I know double mutant epistasis is always downstream> upstream but if upstream gene controls downstream, what happens?arrow_forwardYes or no? during in situ hybridization for signal development the beta galactosidase is used.arrow_forwardLet’s suppose a researcher was interested in the effects of mutationson the expression of a protein-encoding gene for a proteinthat is 472 amino acids in length. This protein is expressed in leafcells of Arabidopsis thaliana. It has a molecular mass of approximately56,640 Da. Make a drawing that shows the expected resultsof a Western blot using proteins isolated from the leaf cells thatwere obtained from the following plants:Lane 1. A plant homozygous for a nonmutant geneLane 2. A plant homozygous for a deletion that removes the promoterfor this geneLane 3. A heterozygous plant in which one gene is nonmutant andthe other gene has a mutation that introduces an early stop codon atcodon 112Lane 4. A plant homozygous for a mutation that introduces anearly stop codon at codon 112Lane 5. A plant homozygous for a mutation that changes codon108 from a phenylalanine codon into a leucine codonarrow_forward
- You are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a mutagenesis screen to identify mutants in the photoperiod pathway. You conduct the screen and find two different plants that show the same mutant phenotype. You then use a complementation test. What is the predicted outcome of this test if both phenotypes are caused by mutations in separate genes? recover the wild type phenotype overexpress the gene O recover the mutant phenotypearrow_forward12. Consider the following genetically controlled biosynthetic pathway in a hypothetical plant: gene A gene B gene C Enzyme A Enzyme B Enzyme C PO → P1 P2 P3 Protein C eme D Assume that gene A controls the conversion of a white pigment, PO, to another white pigment, P1; the dominant allele A codes for the enzyme necessary to catalyze this conversion, but the recessive allele a codes for a defective enzyme (with no activity). Gene B controls the conversion of the white pigment, P1, to a pink pigment, P2; again, the dominant allele, B, produces the enzyme necessary for the P1 - P2 conversion, but the recessive allele b produces an inactive product. The dominant allele, C, of a third gene codes for an enzyme that catalyzes the conversion of the pink pigment, P2, to a red pigment. P3; its recessive allele, c, produces an altered enzyme with no activity. The dominant allele, D, of a fourth gene produces a gene product that completely inhibits the activity of enzyme C; that is, it blocks the…arrow_forwardthe photos below show flowers from two Arabidopsisplants. The plant on the left is wild-type (unmutated); theother carries a mutation that causes its flowers to havesepals and petals instead of stamens and carpels. Themutation inactivated one of the plant's ABC floral identity genes. Refer to Figure 10.8 and decide which gene(A, B, or C) has been inactivatedarrow_forward
- You conduct an experiment to study the expression of the S protein through the tissues of your favorite plant (Arabidopsis thaliana). The morning of the experiment you inject in the leaves a messenger RNA that codes for the synthesis of a single protein made of two parts that are attached to each other: the functional S protein and a red fluorescent protein tag (RFP). You perform two cross sections of the same root, one section at the start of the experiment (time = Oh; corresponding to the time of injection) and one section in the afternoon (time = 8h). Through fluorescent microscopy you observe a change in the coloration inside the cells of the root's central tissues (as indicated by the arrows): from no coloration (time = Oh) to red (time = 8h). • The cells of the central tissues in the roots do not have nuclei or ribosomes. How can you explain this change of coloration? Please provide a cellular feature that can lead to this. • What is one advantage of using an RFP-tag in an mRNA?…arrow_forwardYou are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a mutagenesis screen to identify mutants in the photoperiod pathway. Given what you know about photoperiodism in Arabidopsis, what phenotype are you looking for and under what photoperiodic conditions would you perform the experiment? delayed flowering in long days delayed flowering in short days same flowering in short days early flowering in short days same flowering in long days early flowering in long daysarrow_forwardQ6. The bacterium Rhizobium radiobacter is well known to biologists because it has the rather astonishing ability to cause natural genetic engineering. That is, several genes are moved from the bacterial cells directly into the nucleus of cells of many higher plant species. Expression of these transferred genes in the infected plants stimulates cell division, creating a mass of undifferentiated tissue. Which of the following signals on these transferred genes could be recognized in the plant host cell but not in the original Rhizobium cell? “TATA” box transcription terminator hairpin loop intron splice signals SD box A. 1, 2 and 3 B. 1 and 3 C. 2 and 4 D. 4 only E. All of 1, 2, 3 and 4arrow_forward
- Please help? me???arrow_forwardTrue or false? wild type fast plants propagate mostly via self pollination transfected beta-galactosidase requires antibiotics to be expressed properly. the bradford assay allows you to determine the concentration of proteins produced fromtransfected DNAarrow_forward4e. You also study the expression of 3 different mutants for this gene. For each mutant answer the following: Does this mutation change the sequence of the protein produced? Why or why not? If it does change the sequence of protein be sure to write out the new sequence. If it does not change the protein sequence, what effect (if any) would you expect it to have on expression of the gene? 1 20 ORI 40 60 5'..TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTATC...3’ 3'...AAGCTCGAGAGCAGCAGCTCTATGCGCTACTATAATGACCATTATACCCCTACGTGATAG...5’ promoter i. Mutant A has a single base pair substitution with the T/A being replaced with C/G base pair at position 35 (position denoted by the * in the sequence above). ii. Mutant B has a 2 G/C pairs inserted between position 19 and 20 (position denoted by the ^ in the sequence above).arrow_forward
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