1 SEM CARDLESS ACC W/RAVEN TEXT
1 SEM CARDLESS ACC W/RAVEN TEXT
12th Edition
ISBN: 9781265321062
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 31, Problem 4S
Summary Introduction

To determine: Choice between fungicide treated pea-seed stock versus the untreated stock, for a crop.

Introduction: A fungus is a eukaryotic organism with thousands of species. Many of these species have the ability to act as a pathogen for plants and as a result, can feed on plants. A fungicide is a drug or medicine that can prevent these fungi growing in the plant species.

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You have discovered a secondary metabolite made by an unusual fungus that has great industrial potential. Unfortunately, the fungus makes very little of this product, so you must develop a scheme to increase the yield. Can you think of using a technique that might be useful to enhance the productivity of that secondary metabolite?
In 1971, Dr. Akira Endo, working at the Sankyo company in Tokyo speculated that fungi not only contained antibiotics but also inhibitors of other processes such as cholesterol metabolism. Over a period of several years, he screened more than 6,000 fungal cultures until a positive result emerged. This compound, which was named compactin, came from the blue-green mold Penicillium citrinum Pen-5 that was isolated from a rice sample collected at a grain shop in Kyoto. Based on biochemical assays, compactin is the first known statin. 1. The original kinetics data for compactin inhibition of HMG-CoA reductase enzyme that was published by Dr. Endo in 1976 is shown on the right. a) Determine the Km and Vmax values for enzymatic activity. Do NOT forget the units. Answers can be expressed either as a fraction or in decimals. With No inhibitor: ; Vmax= -60 1/V, (nanomoles/min)-1 0.4 0.35+ 0.3 0.25 0.2 0.15 0.1 0.05 -40 -20 0 + 24 nM compactin + 70 nM compactin None T 20 40 60 1/[HMG-CoA], (mM)-1…
Indoor plants have poor arbuscular mycorrhizal colonization as compare to plants grown in open area. Suggest the possible reason for this.
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