1 SEM CARDLESS ACC W/RAVEN TEXT
12th Edition
ISBN: 9781265321062
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 30.5, Problem 2LO
Summary Introduction
To distinguish: Amongberries, legumes, drupes, and samaras.
Introduction: The plants are multicellular, predominantly photosynthetic eukaryotes. They belong to the kingdom Plantae. A flower is a special part of the plant. The plants possess whorls of colorful sepals or petals and bear the reproductive structures such as pistils or stamens that are involved in the development of fruits and seeds.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 30 Solutions
1 SEM CARDLESS ACC W/RAVEN TEXT
Ch. 30.1 - List the evolutionary advantages of seeds.Ch. 30.1 - Distinguish between pollen and sperm in seed...Ch. 30.2 - Prob. 1LOCh. 30.2 - Prob. 2LOCh. 30.3 - Prob. 1LOCh. 30.3 - Prob. 2LOCh. 30.3 - Prob. 3LOCh. 30.4 - Describe four ways in which seeds help to ensure...Ch. 30.4 - List environmental conditions that can lead to...Ch. 30.5 - Prob. 1LO
Ch. 30.5 - Prob. 2LOCh. 30 - Using the graph in figure 30.13, calculate the...Ch. 30 - Prob. 1IQCh. 30 - Prob. 2IQCh. 30 - Prob. 3IQCh. 30 - Prob. 4IQCh. 30 - The lack of seeds is a characteristic of all a....Ch. 30 - Prob. 2UCh. 30 - Which of the following gymnosperms possesses a...Ch. 30 - In a pine tree, the microspores and megaspores are...Ch. 30 - Which of the following terms is NOT associated...Ch. 30 - Which of the following potentially represents the...Ch. 30 - Prob. 7UCh. 30 - Prob. 8UCh. 30 - The pericarp is the a. ovary wall. b. developing...Ch. 30 - Reproduction in angiospenns can occur more quickly...Ch. 30 - In double fertilization, one sperm produces a...Ch. 30 - Apply your understanding of angiosperms to...Ch. 30 - Comparing stems of two plant specimens under the...Ch. 30 - Prob. 5ACh. 30 - Fruits are complex organs that are specialized for...Ch. 30 - You haw been hired as a research assistant to...Ch. 30 - Prob. 2SCh. 30 - The relationship between flowering plants and...Ch. 30 - Prob. 4S
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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