The principle of micellar electrokinetic capillary chromatography and its difference from capillary zone electrophoresis (CZE) needs to be explained. Concept introduction: Micellar electro kinetic capillary chromatography or MEKC is modified capillary electrophoresis wherein the samples are separated by partitioning the micelles and buffer solution. In this surfactant is added to buffer to get micelles. Micelle consists of conglomeration of 40 and above surfactant molecules in which the hydrocarbon tail which is positive points inward while the negative charged head point outwards. The capillary zone electrophoresis involves buffer solution and sample loaded is placedin additional buffer solution. The capillary tube containing the sample is anode and solute migrates towards cathode at velocity.

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Chapter 30, Problem 30.7QAP

Interpretation Introduction

Interpretation:

The principle of micellar electrokinetic capillary chromatography and its difference from capillary zone electrophoresis (CZE) needs to be explained.

Concept introduction:

Micellar electro kinetic capillary chromatography or MEKC is modified capillary electrophoresis wherein the samples are separated by partitioning the micelles and buffer solution. In this surfactant is added to buffer to get micelles. Micelle consists of conglomeration of 40 and above surfactant molecules in which the hydrocarbon tail which is positive points inward while the negative charged head point outwards.

The capillary zone electrophoresis involves buffer solution and sample loaded is placedin additional buffer solution. The capillary tube containing the sample is anode and solute migrates towards cathode at velocity.

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