EBK PRINCIPLES OF BIOLOGY
EBK PRINCIPLES OF BIOLOGY
2nd Edition
ISBN: 8220103675338
Author: BROOKER
Publisher: YUZU
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Chapter 30, Problem 2TY
Summary Introduction

Introduction:

Soil is a rich source of nutrients for plants. Depending on the concentration and the combination of mineral nutrients available in the soil, plant growth occurs. Plants use inorganic minerals for nutrition. For example- sulfur is converted to sulfate; atmospheric nitrogen is fixed by the soil so that roots can take it. There are 2 classes of nutrients, which are required for the growth of the plants: micronutrients and macronutrients.

Micronutrients are needed in a very small amount and often required as the enzyme’s cofactors. The name of some micronutrients is zinc, iron, copper, and manganese.

Macronutrients are needed in large quantities. They are known as the building blocks of the cellular components. Carbon, Oxygen, Hydrogen, Nitrogen, Phosphorus, etc are considered as the macromolecules.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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