Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 30, Problem 2Q
Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that would result from digesting pLAB30 with EcoR1, with BamHI, and with both enzymes together.
Which enzyme will give the smallest piece containing the tetracycline-resistance gene?
Expert Solution & Answer
Summary Introduction
To determine:
The size and number of DNA Fragments given by restriction enzymes Eco RI, Bam HI, and both these enzymes together.
Introduction:
Restriction enzyme is an enzyme that cuts the DNA fragment at the specific site by recognizing the DNA sequence at that site. The site at which the DNA is cut is termed as the restriction site.
Explanation of Solution
pLAB30 is an 18 kb map, which is cut by two restriction enzymes, Eco RI, and Bam HI. When Eco RI acts, then it cuts the DNA fragment at two sites, giving an 11 kb fragment and 7 kb fragment of the DNA.
When Bam HIacts, then it cuts DNA fragment at three sites, giving three fragments. The size of these fragments is 9 kb, 3 kb, and 6 kb.
When both these restriction enzymes act together, then 5 DNA fragments are obtained. The size of these fragments is 1 kb, 3 kb, 3 kb, 3kb, and 8 kb.
Conclusion
The table showing the size and number of DNA fragments after the DNA is acted upon by restriction enzymes is shown below:
| Restriction enzyme | Number of DNA fragments obtained | Size of DNA fragments |
| Eco RI | 2 | 11 kb and 7 kb |
| Bam HI | 3 | 9 kb, 3 kb, and 6 kb |
| Eco RI + Bam HI | 5 | 1 kb, 3 kb, 3 kb, 3kb, and 8 kb |
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Chapter 30 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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