Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 30, Problem 2CT
Summary Introduction
To determine:
The advantages and disadvantages of RFLP with comparison to conventional biochemical testing.
Introduction:
Restriction fragment length polymorphism, RFLP, is a technique used in molecular biology to exploit a change in DNA fragments done by restriction enzymes. RFLPs are used as DNA markers for genetic maps.
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8. Transcription and Translation Practice: (Video 10-1 and 10-2)
A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense
DNA strand and label the 5' and 3' ends.
B. Use the antisense strand that you create in part A as a template to create the mRNA
transcript of the gene and label the 5' and 3' ends.
C. Translate the mRNA you produced in part B into the polypeptide sequence making sure
to follow all the rules of translation.
5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand)
4
Chapter 30 Solutions
Laboratory Experiments in Microbiology (11th Edition)
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