Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
12th Edition
ISBN: 9780135188996
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 3, Problem 9E.3RQ
Summary Introduction

To determine: Why nigrosin stain does not penetrate the bacterial cells.

Introduction: There are various staining methods that are used for staining the bacteria, namely simple staining, Gram-staining, negative staining, spore staining, and so on. In simple staining methods, two stains are widely used namely acidic stain and basic stain. Examples of acidic stains are picric acid, eosin, acid fuschin, and so on, and for basic stains, they are methylene blue, safranin, and crystal violet.

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