Chapter 3, Problem 3.5.3C

true or false: DNA polymerase III performs nick translation.

You are a forensic scientist in charge of DNA samples in a case where a man was murdered. You conduct gel electrophoresis on the samples to determine the DNA profile of the samples. The rows of bands have been marked to make it easier to reference the bands. The sample marked 'evidence' came from under the victim's fingernails, likely indicating a struggle. There are two suspects in the case, both of whom are brothers, they are marked suspect 1 and suspect 2, for brother 1 and brother 2, respectfully. Does the evidence collected match the DNA profiles of either brother? If yes, which one? If no, then would it be likely that the actual criminal is a family member of the brothers? If yes, what could the relation be (mom, dad, cousin, aunt, or uncle)? Note: there is a sister in their family, their parents are both still alive, and they have a robust extended family. The brothers are not twins.

Think of a possible safety or ethical issue related to genetic engineering and discuss briefly (in 3-5 sentences) why this is a valid concern. Write your answer on the space provided below.

Using suitable illustrations describe a method to sequence a fragment of DNA and include how the same method can be used to sequence RNA.

schematic diagram for the procedure in doing DNA extraction, isolation and quantification

name the enzyme which is used to combine or glue two different molecules of a fregment of the DNA

Suggest and describe two methods to detect DNA concentration by using in text citation and provide the references with APA format. without the UV spectrophotometer method

DNA fragments can be separated and identified by(gas chromatography, capillary electrophoresis).

give the significance/role/effect of the reagent/condition in the isolation or analysis of a biomolecule. using citrate buffer (ph 4.5) instead of Tris-HCl buffer (ph 7.5) during the DNA isolation