Strategies for the purification of an antibody out of antiserum found after inoculation of antigen needs to be proposed. Concept introduction: Biochemists produce antibodies which is specific to certain antigen, by injecting that antigen into a rabbit twice three weeks apart. This antigen stimulates the production of antibodies in the blood which recognize the antigen. Then blood from the rabbit is drawn out and blood serum which contains the antibodies is separated from blood cells from centrifugation. Then antibodies are purified by various strategies for further use because cell may a produce different antibodies for different surface features of the same antigen. These antibodies are referred to as polyclonal antibodies.

Question

Want to see the full answer?

Answer 1

Question

Chapter 3, Problem 30P

Interpretation Introduction

Interpretation:

Strategies for the purification of an antibody out of antiserum found after inoculation of antigen needs to be proposed.

Concept introduction:

Biochemists produce antibodies which is specific to certain antigen, by injecting that antigen into a rabbit twice three weeks apart. This antigen stimulates the production of antibodies in the blood which recognize the antigen. Then blood from the rabbit is drawn out and blood serum which contains the antibodies is separated from blood cells from centrifugation. Then antibodies are purified by various strategies for further use because cell may a produce different antibodies for different surface features of the same antigen. These antibodies are referred to as polyclonal antibodies.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

In 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat liver. Their hypothesis was that phosphocholine joined with some cellular component to yield lecithin. In an earlier experiment, incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled lecithin. This became their assay for the enzymes involved in lecithin synthesis. Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values. -O-P-O-CH2-CH₁₂-N(CH3)3 Phosphocholine H₂C-O-C-R HC-O-C-R2 + + + Cell fraction + ? HC-O-P-O-CH₁₂-CH₂-N(CH), O Phosphatidylcholine The researchers then centrifuged the broken cell preparation to separate the membranes from the soluble proteins. They tested three preparations: whole extract, membranes, and soluble proteins. Table 1 summarizes the results. Table 1: Cell fraction requirement for incorporation of 32p-phosphocholine into…

Researchers isolated an unknown substance, X, from rabbit muscle. They determined its structure from the following observations and experiments. (a) Qualitative analysis showed that X was composed entirely of C, H, and O. A weighed sample of X was completely oxidized and the H2O and CO2 produced were measured. This quantitative analysis revealed that X contained 40.00% C, 6.71% H, and 53.29% O by weight. (b) The molecular mass of X, as determined by mass spectrometry, was 90.00 atomic mass units (u). (c) Infrared spectroscopy showed that X contained one double bond. (d) X dissolved readily in water, and the solution demonstrated optical activity when tested in a polarimeter. (e) The aqueous solution of X is acidic. What is the empirical formula of X?

Show work. don't give Ai generated solution....give correct solution