Chapter 3, Problem 28P

Interpretation Introduction

Interpretation:

The sequence of the peptide consisting of 14 amino acids should be determined.

Concept introduction:

Trypsin cleaves peptide chains at the carboxyl side of the amino acids lysine (K) or arginine (R) if they are not followed by proline. Carboxypeptidase hydrolyzes the first peptide bond at the C-terminal of the peptide chain except for arginine (R), lysine (K) or proline (P). Chymotrypsin cleaves the peptide bond at the C-terminal where there ishydrophobic amino acids like tyrosine (Y), tryptophan (W), phenylalanine (F), leucine (L) and methionine (M). Cyanogen bromide hydrolyzes peptide bond at C-terminal of methionine residues. Homoserine is a product of cyanogen bromide cleavage by degradation of methionine.