Chapter 3, Problem 20E

Summary Introduction

To determine: The restriction enzymes would not interfere with the ampicillin resistance or production of β-galactosidase by the host cell.

Concept introduction: The plasmid pUC18 is a circular plasmid that contains multiple restriction sites. In pUC18, the enzyme β-galactosidase is encoded by the lacZ gene. The β-galactosidase cleaves the colorless compound X-gal to a blue-colored product.